Detecting QTL for internode length in rice to improve lodging resistance of high-quality japonica cultivar

文献类型： 外文期刊

第一作者： Zhao, Mingzhu

作者： Zhao, Mingzhu;Wang, Lili;Ma, Zuobin;Tang, Zhiqiang;Zheng, Wenjing;Zhao, Mingzhu

作者机构：

关键词： Rice (Oryza sativa); Internode length; Lodging resistance; DEP1; Grain quality

期刊名称：EUPHYTICA （ 影响因子：1.6； 五年影响因子：1.8 ）

ISSN： 0014-2336

年卷期： 2024 年 220 卷 7 期

页码：

收录情况： SCI

摘要： Internode length is an important trait to improve the lodging resistance of rice (Oryza sativa), especially for some high-quality japonica cultivars that are highly susceptible to lodging. Yet, there is little information on the genetic basis of internode length in rice. We performed QTL analysis for internode length using a recombinant inbred line (RIL) population derived from the lodging susceptible japonica cultivar Gangyuan8 (GY8) and super rice cultivar Liaoxing1 (LX1). A total of 19 QTLs were detected by individual environmental analyses and multi-environment joint analyses. A stable QTL with major effect was identified q4IL9, and its allele for reduced length originated from the donor LX1. The most likely candidate gene for q4IL9 was DEP1, which encodes a highly cysteine-rich G protein gamma subunit. A mutant dep1 was developed using CRISPR/Cas9 within the genetic background of Daohuaxiang. The mutant dep1 exhibited shorter internode length and reduced plant height as well as a thicker culm wall, which may be characterized by greater lodging resistance. However, several differential metabolites including sucrose, D-(+) -maltose, stearic acid, palmitic acid, and indole-3-acetic acid were identified in grains, resulting in the having less desirable eating quality in mutant dep1. Our results revealed a major-effect QTL for internode length and provided an important strategy for improving lodging resistance in rice, but its negative effect on grain quality requires further investigation.

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