Beta-Sitosterol Enhances Classical Swine Fever Virus Infection: Insights from RNA-Seq Analysis

文献类型： 外文期刊

第一作者： Liu, Yayun

作者： Liu, Yayun;Yin, Dongdong;Wang, Jieru;Dai, Yin;Shen, Xuehuai;Yin, Lei;Pan, Xiaocheng;Liu, Yayun;Yin, Dongdong;Wang, Jieru;Dai, Yin;Shen, Xuehuai;Yin, Lei;Pan, Xiaocheng;Zhou, Bin

作者机构：

关键词： beta-sitosterol (BS); classical swine fever virus (CSFV); lipid compounds; replication; differentially expressed genes (DEGs)

期刊名称：VIRUSES-BASEL （ 影响因子：3.5； 五年影响因子：3.7 ）

ISSN：

年卷期： 2025 年 17 卷 7 期

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收录情况： SCI

摘要： Beta-sitosterol (BS), a naturally occurring phytosterol abundant in plants, has been reported to exhibit diverse biological activities, including immunomodulatory and antiviral effects. Classical swine fever virus (CSFV), a member of the Pestivirus genus, remains a persistent threat to the swine industry worldwide, causing considerable economic damage. Our research found that BS significantly enhances the replication of both the CSFV-Shimen strain and the attenuated C-strain vaccine virus in PK-15 cells. Additionally, transcriptomic profiling (RNA-Seq) identified 175 differentially expressed genes (DEGs) following BS exposure, comprising 53 upregulated and 122 downregulated genes. Further results demonstrated that treatment with beta-sitosterol suppressed I kappa B alpha expression, thereby activating the NF-kappa B pathway, and that knockdown of endogenous I kappa B alpha significantly promoted CSFV replication. These findings contribute to a deeper understanding of how BS influences the CSFV infection process, suggesting its role as a host lipid-associated factor facilitating viral propagation.

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