Label-Free Microchannel Immunosensor Based on Antibody-Antigen Biorecognition-Induced Charge Quenching

文献类型： 外文期刊

第一作者： Feng, Niu

作者： Feng, Niu;Peng, Xuewen;Wang, Zhipan;Chen, Yiping;Yu, Xiaoping;Shentu, Xuping;Chen, Yiping

作者机构：

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：8.008； 五年影响因子：7.528 ）

ISSN： 0003-2700

年卷期： 2022 年 94 卷 48 期

页码：

收录情况： SCI

摘要： Pursuing convenient operations and precise testing have become an urgent requirement in clinical diagnosis, treatment, and prognosis. Label-free detection is desirable for obviating the labeling process while maintaining high sensitivity and efficiency. Here, we used the dual properties of highly selective antibody-antigen recognition and potential signaling of biomolecules to construct a label-free electroosmotic flow-driven microchannel (LF-EMB) biosensor based on an antibody-antigen biorecognition-induced charge quenching theory proposed herein. The LF-EMB consists of a one-step immune-reaction, onebutton portable device, and supporting microfluidic chip, providing a high-powered tool for rapid on-site testing. The LF-EMB quantified interleukin-6 and kanamycin levels down to 1 pg/mL and 5 pg/mL, respectively, with the whole analysis completed within 35 min. The outstanding sensitivity and detection speed of the constructed LF-EMB provide a convenient option for the quantitative detection of inflammatory markers and antibiotics.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序