Molecular structures and functional exploration of NDA family genes respond tolerant to alkaline stress in Gossypium hirsutum L.

文献类型： 外文期刊

第一作者： Fan, Yapeng

作者： Fan, Yapeng;Zhang, Yuexin;Rui, Cun;Zhang, Hong;Xu, Nan;Wang, Jing;Han, Mingge;Lu, Xuke;Chen, Xiugui;Wang, Delong;Wang, Shuai;Guo, Lixue;Zhao, Lanjie;Huang, Hui;Wang, Junjuan;Sun, Liangqing;Chen, Chao;Ye, Wuwei

作者机构：

关键词： NDA; Phylogenetic analysis; Cis-elements; Expression pattern; VIGS; Alkaline stress

期刊名称：BIOLOGICAL RESEARCH （ 影响因子：7.634； 五年影响因子：6.7 ）

ISSN： 0716-9760

年卷期： 2022 年 55 卷 1 期

页码：

收录情况： SCI

摘要： Background The internal NAD(P)H dehydrogenase (NDA) gene family was a member of the NAD(P)H dehydrogenase (ND) gene family, mainly involved in the non-phosphorylated respiratory pathways in mitochondria and played crucial roles in response to abiotic stress. Methods The whole genome identification, structure analysis and expression pattern of NDA gene family were conducted to analyze the NDA gene family. Results There were 51, 52, 26, and 24 NDA genes identified in G. hirsutum, G. barbadense, G. arboreum and G. raimondii, respectively. According to the structural characteristics of genes and traits of phylogenetic tree, we divided the NDA gene family into 8 clades. Gene structure analysis showed that the NDA gene family was relatively conservative. The four Gossypium species had good collinearity, and segmental duplication played an important role in the evolution of the NDA gene family. Analysis of cis-elements showed that most GhNDA genes contained cis-elements related to light response and plant hormones (ABA, MeJA and GA). The analysis of the expression patterns of GhNDA genes under different alkaline stress showed that GhNDA genes were actively involved in the response to alkaline stress, possibly through different molecular mechanisms. By analyzing the existing RNA-Seq data after alkaline stress, it was found that an NDA family gene GhNDA32 was expressed, and then theGhNDA32 was silenced by virus-induced gene silencing (VIGS). By observing the phenotype, we found that the wilting degree of silenced plants was much higher than that of the control plant after alkaline treatment, suggesting that GhNDA32 gene was involved in the response to alkaline stress. Conclusions In this study, GhNDAs participated in response to alkaline stress, especially NaHCO3 stress. It was of great significance for the future research on the molecular mechanism of NDA gene family in responding to abiotic stresses.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序