Based on the TLR4/NLRP3 Pathway and Its Impact on the Formation of NETs to Explore the Mechanism of Ginsenoside Rg1 on Acute Gouty Arthritis

文献类型： 外文期刊

第一作者： Li, Zhiman

作者： Li, Zhiman;Yu, Yang;Huo, Xiaohui;Sha, Jiyue;Qu, Di;Sun, Qiang;Sun, Yinshi;Li, Zhilong

作者机构：

关键词： ginsenoside Rg(1); acute gouty arthritis; neutrophil extracellular traps; inflammatory response

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 9 期

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收录情况： SCI

摘要： This study investigated whether ginsenoside Rg(1) (G-Rg(1)) alleviated acute gouty arthritis (AGA) in rats by modulating the TLR4/NLRP3 pathway and neutrophil extracellular trap (NET) formation. Rats were orally administered G-Rg(1) or colchicine (Col) for 7 days, and monosodium urate (MSU) was injected into the ankle joints on day 5 to induce AGA. Joint swelling, histopathology (HE staining), and serum markers (MPO, NE, MPO-DNA, IL-6, IL-1 beta; ELISA) were assessed at the baseline and 6-36 h post-modeling. Western blot and immunofluorescence analyzed the NET-related and TLR4/NLRP3 pathway proteins in synovial tissue. G-Rg(1) significantly reduced ankle swelling and synovial inflammation compared with the AGA group, lowered the serum IL-6, IL-1 beta, MPO, NE, and MPO-DNA levels, and suppressed NET-associated protein expression. Mechanistically, G-Rg(1) downregulated TLR4/NLRP3 pathway activation in synovial tissue. These findings suggest that G-Rg(1) mitigates AGA by inhibiting TLR4/NLRP3 signaling, thereby reducing inflammatory cytokine release and NET formation.

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