Molecular pathological characteristics and mechanisms of the liver in metabolic disease-susceptible transgenic pigs

文献类型： 外文期刊

第一作者： Du, Juan

作者： Du, Juan;Zhang, Kaiyi;Miao, Jiakun;Yang, Yu;Tian, Yuying;Wu, Tianwen;Tao, Cong;Wang, Yanfang;Yang, Shulin

作者机构：

关键词： Transgenic pigs; ETNPPL; GABBR2; BMP8B; Bis (monoacylglycerol) phosphates; Sulfatides

期刊名称：LIFE SCIENCES （ 影响因子：5.1； 五年影响因子：5.4 ）

ISSN： 0024-3205

年卷期： 2025 年 362 卷

页码：

收录情况： SCI

摘要： Aims: This study aimed to explore the molecular pathological mechanisms of the liver in metabolic disease- susceptible transgenic pigs via multiomics analysis. Materials and methods: The triple-transgenic ( PNPLA3 I148M-GIPR dn-hIAPP ) pig model (TG pig) was successfully constructed in our laboratory via the CRISPR/Cas9 technique previously described. Wild-type (WT) pigs and TG pigs after 2 or 12 months of high-fat and high-sucrose diet (HFHSD) induction (WT2, TG2, WT12, and TG12 groups, respectively) were used as materials. The transcriptome, metabolome, and lipidome were used to investigate the molecular mechanisms of the liver in pigs. Key findings: The TG2 pigs presented mild metaflammation and insulin resistance (IR) which was similar to WT12 pigs. Compared with the other three groups, the TG12 pigs presented severe hepatocyte ballooning, fat deposition, and portal area fibrosis. The transcriptome data suggested that the TG2 pigs presented upregulated gene expression in the extracellular matrix (ECM). The TG12 pigs presented more severe metaflammation and exhibited imbalanced glycolipid metabolism. Interestingly, genes such as ETNPPL, GABBR2, and BMP8B might be key regulatory targets for liver injury. The metabolome and lipidome suggested that long-chain polyunsaturated fatty acids (LCPUFAs) and phospholipids with corresponding LCPUFAs were remodelled. Importantly, bis(monoacylglycerol) phosphates (BMPs) and sulfatides (SLs) could be the key regulatory metabolites in liver injury. Significance: ETNPPL, GABBR2, and BMP8B might be potential therapeutic targets for liver injury. BMPs and SLs might be biomarkers for the diagnosis and treatment of liver diseases.

分类号：

  • 相关文献
作者其他论文 更多>>
  • A rapid and highly sensitive CRISPR assay utilizing Cas12a orthologs for the detection of Novel Duck Reovirus

    作者：Wang, Yuan;Fu, Lanting;Li, Sheng;Tao, Dagang;Ruan, Jinxue;Xie, Shengsong;Wang, Yuan;Fu, Lanting;Li, Sheng;Tao, Dagang;Ruan, Jinxue;Xie, Shengsong;Wang, Cui;He, Daqian;Gong, Ping;Yang, Yu

    关键词：NDRV; Cas12a orthologs; Gs12-16; Gs12-18; CRISPR-based diagnostics

  • Ethanol inhibited growth hormone receptor-mediated endocytosis in primary mouse hepatocytes

    作者：Ge, Zihan;Liu, Xingjie;Zhang, Wei;Xian, Li;Li, Ruonan;Ge, Zihan;Yang, Yu;Lan, Hainan;Zhang, Yawen

    关键词：growth hormone; growth hormone receptor; ethanol; nuclear translocation; endocytosis

  • Establishment and identification of spleen cell line from tiger puffer fish (Takifugu rubripes) and immune transcriptome response to IHNV

    作者：He, Yangbin;Zhang, Jingjing;Meng, Bo;He, Yangbin;Zhang, Jingjing;Lin, Lei;Meng, Bo;Dong, Caichao;Liu, Yuyan;Yang, Yu;Ji, Honglong;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Liu, Yuyan;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Liu, Yuyan;Liu, Kaiqiang;Wang, Qian;Shao, Changwei

    关键词：Tiger puffer fish; Spleen; Cell line; Immunology; Transcriptome

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词：metagenome; microbiome; microbiota; pipeline; visualization

  • Enhancing alfalfa and sorghum silage quality using agricultural wastes: fermentation dynamics, microbial communities, and functional insights

    作者：Zhao, Yuanyuan;Chen, Yulian;Lei, Yao;Li, Maoya;Wang, Jiachuhan;He, Xiangjiang;Yang, Yu;Zhang, Xinyi;Cheng, Qiming;Chen, Chao;Liu, Dianpeng;Liu, Shengnan;Zhang, Xiaoqing;Cheng, Qiming

    关键词：Lactobacillus acidophilus; Rosa roxburghii pomace; Alfalfa; Bacterial community; Moutai distillers' grain; Sorghum

  • Microbial Community Structure and Metabolic Potential Shape Soil-Mediated Resistance Against Fruit Flesh Spongy Tissue Disorder of Peach

    作者：Chen, Weifeng;Tang, Dan;Huang, Jia;Yang, Yu;Zhang, Liangbo;Chen, Weifeng;Tang, Dan;Huang, Jia;Yang, Yu;Zhang, Liangbo

    关键词：peach; fruit flesh spongy tissue disorder; soil physicochemical properties; rhizosphere microbiome; plant growth-promoting microbes; metagenomics

  • Single-nucleus RNA sequencing defines adipose tissue subpopulations that contribute to Tibetan pig cold adaptation

    作者：Liu, Jiali;Chen, Chuanhe;Chui, Linya;Zhang, Xueping;Wang, Xiao;Yang, Chunhuai;Wu, Tianwen;Yang, Shulin;Tao, Cong;Wang, Yanfang;Jiang, Yao;Liu, Tianxia;Cui, Along;Zhang, Xueping;Wang, Yu;Zhang, Ying;Zhao, Jianguo;Liu, Tianxia;Wang, Yu;Zhao, Jianguo;Liu, Tianxia;Cui, Along;Wang, Yu;Zhang, Ying;Zhao, Jianguo;Zhang, Ying;Zhao, Jianguo;Cui, Along;Zhang, Xueping;Chui, Linya;Huang, Jiaojiao;Zhao, Jianguo;Wang, Yanfang

    关键词：Pig; snRNA-seq; Adipocytes; Subpopulations; Cold stimulation

微信小程序