Copper-based nanozyme-linked immunosorbent assay for quantitative detection of fumonisin B1

文献类型： 外文期刊

第一作者： Tang, Fen

作者： Tang, Fen;Guo, Qi;Fu, Lina;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Zhang, Zhaowei;Li, Peiwu;Li, Peiwu

作者机构：

关键词： Copper-based nanozyme; Nanozyme-linked immunosorbent assay; Fumonisin B 1; Peroxidase-like activity; Cereals

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.6； 五年影响因子：4.6 ）

ISSN： 0889-1575

年卷期： 2025 年 138 卷

页码：

收录情况： SCI

摘要： Fumonisin B1 (FB1) is a Group 2B carcinogen. It is vital to develop an ultra-sensitive, low-cost, and rapid screening method for FB1 for food safety risk control. In this study, a novel copper-based nanozyme was synthesized. The anti-FB1 monoclonal antibody (mAb) was conjugated with copper-based nanozyme through physical adsorption to probe construction. The probe could compete with the limited antigen and trigger a color change when employing the 3,3',5,5'-tetramethylbenzidine (TMB) as chromogenic substrate in the presence of H2O2. Several experimental conditions were optimized, including the concentration of coating antigen, the concentration of mAb coupled to the nanozyme, incubation time, and methanol concentration. Under optimal conditions, a direct competitive nanozyme-linked immunosorbent assay (NLISA) has been established with a limit of detection (LOD) of 6.52 pg/mL. The developed method presented good accuracy and precision. The spiked recoveries ranged from 97.368 % to 120.916 %, with coefficient of variation (CV) below 10 %. In addition, there was a high consistency (R2=0.9820 for corn; R2=0.9936 for wheat) between the developed detection method and the reference method, liquid chromatography/mass spectrometry (LC-MS/MS). This research can expand the application areas of copper-based nanozymes and improve the sensitivity and efficiency of FB1 monitoring.

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