Melatonin regulates dihydrotestosterone formation via its membrane receptor in the epididymal epithelial cells of sheep

文献类型： 外文期刊

第一作者： Duan, Hongwei

作者： Duan, Hongwei;Ge, Wenbo;Wu, Jianxin;Lv, Jianshu;Li, Zongshuai;Dong, Weitao;Du, Xianghong;Zhang, Lihong;Zhang, Yong;Hu, Junjie;Zhao, Xingxu;Ge, Wenbo;Hu, Junjie;Zhao, Xingxu

作者机构：

关键词： Dihydrotestosterone; Melatonin; Regulatory mechanism; Sheep epididymis

期刊名称：THERIOGENOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 0093-691X

年卷期： 2023 年 198 卷

页码：

收录情况： SCI

摘要： Both melatonin and androgen, which affect sperm fertility, are the important factors in epididymis of male animal. In the present study, we confirmed that melatonin regulates the formation of dihy-drotestosterone (DHT) in sheep epididymides. Here, we investigated the localization and the expression levels of melatonin keys synthases AANAT and HIOMT, membrane receptors MT1 and MT2, and nuclear receptor RORa in sheep epididymides and testes. We also cultured epididymal epithelial cells and treated them with different concentrations of melatonin (10-11-10-7 M) and luzindole (10-5 M) and 4P-PDOT (10-5 M) to investigate whether melatonin is involved in the regulation of DHT formation and whether these effects are mediated through its receptor pathways. The results showed that AANAT, HIOMT, MT1, MT2, and RORa were differentially expressed between sheep epididymides and testes. In addition, melatonin is involved in mediating the formation of DHT in epididymal epithelial cells, and its influence on DHT is at least partially regulated by the melatonin receptor pathway. Our findings showed that melatonin regulates the functions of the testes and epididymides through an autocrine mechanism and regulates the formation of androgen in sheep epididymides via the receptor pathway. These results provide a basis for further exploring the regulatory mechanisms of melatonin in animal reproduction.(c) 2022 Elsevier Inc. All rights reserved.

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