Disease resistance of Brassicajuncea to Sclerotinia sclerotiorum is established through the induction of indole glucosinolate biosynthesis

文献类型： 外文期刊

第一作者： Zhang, Jinze

作者： Zhang, Jinze;Yang, Xu;Jin, Hairun;Yu, Kunjiang;Xiao, Lijing;Ouyang, Qingjing;Tian, Entang;Jiang, Yingfen

作者机构：

关键词： Sclerotinia sclerotiorum; Sclerotinia stem rot; Resistance gene; Molecular mechanism

期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：2.8； 五年影响因子：2.8 ）

ISSN： 0885-5765

年卷期： 2024 年 133 卷

页码：

收录情况： SCI

摘要： Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum, is the main disease threat to oilseeds in Brassiceae, causing significant yield losses and reduction in oil content and quality. While the genetic mechanism underlying its lethal phenotype, particularly in B. juncea, remains largely unexplored. Transcriptome analysis revealed a large number of defense-related genes and response processes in B. napus and B. oleracea. However, similarities and differences in the defense responses to S. sclerotiorum on B. juncea are rarely reported. In the present study, we reported a B. juncea breeding line of H83 with high S. sclerotiorum resistance, which was used for transcriptome analysis compared to L36 with low resistance. A novel regulatory network was proposed to defend against S. sclerotiorum invasion in B. juncea. Upon infection of S. sclerotiorum, a series of were initiated within 12 h, and then defenses were activated to restrict the development and spread of S. sclerotiorum by inducing the massive synthesis of indole glucosinolates after 24 h. Twelve hub genes involved in the network were identified by the weighted gene co-expression network (WGCNA), which are involved in plant-pathogen interaction, signaling pathway genes, indole glucosinolate biosynthesis and cell wall formation. The hub genes were further validated by qRT-PCR. The research revealed a new resistant line of H83 against S. sclerotiorum and a different regulatory network in B. juncea, which would be beneficial for the future effective breeding of Sclerotiniaresistant varieties.

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