Comparative Genomic Analysis of the Thiolase Family and Functional Characterization of the Acetyl-Coenzyme A Acyltransferase-1 Gene for Milk Biosynthesis and Production of Buffalo and Cattle

文献类型： 外文期刊

第一作者： Deng, Tingxian

作者： Deng, Tingxian;Wu, Jiyun;Wang, Xiaojie;Shaukat, Aftab;Zhou, Xiang;Zhou, Yang;Sun, Hui;Wei, Pengfei;Sun, Nan;Huang, Qianzhi;Xu, Linghua;Liu, Miaoyu;Lin, Yuxin;Yang, Liguo;Hua, Guohua;Deng, Tingxian;Wu, Jiyun;Sun, Hui;Hua, Guohua;Wu, Jiyun;Sun, Hui;Hua, Guohua;Abdel-Shafy, Hamdy;Shaukat, Aftab;Yang, Liguo;Hua, Guohua

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关键词： ACAA1; cattle; buffalo; mammary epithelial cells; cell proliferation; milk production traits

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 7 期

页码：

收录情况： SCI

摘要： Cattle and buffalo served as the first and second largest dairy animals, respectively, providing 96% milk products worldwide. Understanding the mechanisms underlying milk synthesis is critical to develop the technique to improve milk production. Thiolases, also known as acetyl-coenzyme A acetyltransferases (ACAT), are an enzyme family that plays vital roles in lipid metabolism, including ACAT1, ACAT2, ACAA1, ACAA2, and HADHB. Our present study showed that these five members were orthologous in six livestock species including buffalo and cattle. Transcriptomic data analyses derived from different lactations stages showed that ACAA1 displayed different expression patterns between buffalo and cattle. Immunohistochemistry staining revealed that ACAA1 were dominantly located in the mammary epithelial cells of these two dairy animals. Knockdown of ACAA1 inhibited mammary epithelial cell proliferation and triglyceride and beta-casein secretion by regulating related gene expressions in cattle and buffalo. In contrast, ACAA1 overexpression promoted cell proliferation and triglyceride secretion. Finally, three novel SNPs (g.-681A>T, g.-23117C>T, and g.-24348G>T) were detected and showed significant association with milk production traits of Mediterranean buffaloes. In addition, g.-681A>T mutation located in the promoter region changed transcriptional activity significantly. Our findings suggested that ACAA1 play a key role in regulating buffalo and cattle milk synthesis and provided basic information to further understand the dairy animal lactation physiology.

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