Genome-wide identification and expression analysis of terpene synthases in Gossypium species in response to gossypol biosynthesis

文献类型： 外文期刊

第一作者： Mehari, Teame Gereziher

作者： Mehari, Teame Gereziher;Fang, Hui;Feng, Wenxiang;Han, Jinlei;Wang, Kai;Wang, Baohua;Zhang, Yuanyuan;Umer, Muhammad Jawad;Liu, Fang;Ditta, Allah;Khan, Muhammad K. R.

作者机构：

关键词： Gossypium; Terpene synthase (TPS); Gossypol; RT-qPCR; Virus-induced gene silencing (VIGS)

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：2.9； 五年影响因子：3.3 ）

ISSN： 1438-793X

年卷期： 2023 年 23 卷 2 期

页码：

收录情况： SCI

摘要： Cottonseed is an invaluable resource, providing protein, oil, and abundant minerals that significantly contribute to the well-being and nutritional needs of both humans and livestock. However, cottonseed also contains a toxic substance called gossypol, a secondary metabolite in Gossypium species that plays an important role in cotton plant development and self-protection. Herein, genome-wide analysis and characterization of the terpene synthase (TPS) gene family identified 304 TPS genes in Gossypium. Bioinformatics analysis revealed that the gene family was grouped into six subgroups TPS-a, TPS-b, TPS-c, TPS-e, TPS-f, and TPS-g. Whole-genome, segmental, and tandem duplication contributed to the evolution of TPS genes. According to the analysis of selection pressure, it was predicted that TPS genes experience predominantly negative selection, with positive selection occurring subsequently. RT-qPCR analysis in TM-1 and CRI-12 lines revealed GhTPS48 gene as the candidate gene for silencing experiments. To summarize, comprehensive genome-wide studies, RT-qPCR, and gene silencing experiments have collectively demonstrated the involvement of the TPS gene family in the biosynthesis of gossypol in cotton.

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