Comprehensive analysis of fatty acid hydroxylase genes reveals GhFAHS11 affecting cotton anther fertility

文献类型： 外文期刊

第一作者： Cao, Xiaocong

作者： Cao, Xiaocong;Li, Jinzhu;Wei, Jingyan;Wang, Huiying;He, Jie;Zhang, Xinjie;Yang, Lu;Liu, Yuanyuan;Wang, Yuan;Zhu, Ruonan;Tang, Shurong;Li, Zhanshuai;Peng, Jun;Li, Jinzhu;Liu, Yongming;Wang, Huiying;He, Jie;Zhang, Xinjie;Yang, Lu;Liu, Yuanyuan;Wang, Yuan;Zhu, Ruonan;Li, Zhanshuai;Peng, Jun;Cao, Xiaocong

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关键词： Cotton; GhFAHS11; FA_hydroxylase; Cuticle wax; Male fertility

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 227 卷

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收录情况： SCI

摘要： The cuticular wax of terrestrial plants' outer epidermis is indispensable in plant reproductive development and response to external environmental stress. Alkanes are the main component of cuticle wax and fatty acid hydroxylases play a key role in alkane biosynthesis; however, their function in cotton remains elusive. Here, 53, 55, 28 and 28 candidate fatty acid hydroxylase superfamily (FAHS) genes in four cultivated cotton species were identified. The FAHSs share relatively conserved gene tructure and motifs. We conducted a systematic structural, expression and functional regulatory analysis of the FAHS genes in cotton. Transcriptome analysis indicated that GhFAHS11 is highly expressed specifically in reproductive organs, especially stamens. Sequence analysis revealed that GhFAHS11 and AtCER1 share conserved histidine-rich domains, LEGW motif and seven trans- membrane domains. Yeast two-hybrid analyses showed that GhFAHS11, GhFAR3 and GhCER2 are all subcellularly localized to the endoplasmic reticulum (ER) and interacted with each other. Moreover, GhFAHS11 is co-expressed with CER3 and CYTB5, involved in cuticular wax biosynthesis. When GhFAHS11 transcription was reduced in cotton, anther dehiscence was blocked, pollen grains were depressed, and pollen activity was reduced. These findings indicate that GhFAHS11 affects the biosynthesis of cotton anther cuticular wax, which regulates the development of anther and pollen, ultimately affecting male fertility. This study provides a reference for the biosynthesis of cotton cuticle wax and its effects on reproductive development and environmental stress responses.

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