Elimination of the yellow pigment gene PSY-E2 tightly linked to the Fusarium head blight resistance gene Fhb7 from Thinopyrum ponticum

文献类型： 外文期刊

第一作者： Li, Xuefeng

作者： Li, Xuefeng;Li, Dong;Xuan, Yu;Hao, Yongchao;Ge, Wenyang;Xu, Shoushen;Hou, Bingqian;Wang, Biao;Liu, Wenwen;Li, Mingzhu;Han, Yi;Bo, Cunyao;Bao, Yinguang;Wang, Hongwei;Kong, Lingrang;He, Ziming;Qi, Zengjun;Zhao, Lanfei;Bai, Guihua;Guo, Jun;Xu, Steven S.;Bai, Guihua

作者机构：

关键词： Triticum aestivum; Thinopyrum ponticum; Fusarium head blight; Fusarium crown rot; Truncated Fhb7 translocation

期刊名称：CROP JOURNAL （ 影响因子：6.6； 五年影响因子：6.5 ）

ISSN： 2095-5421

年卷期： 2023 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Fhb7 is a major gene that was transferred from Thinopyrum ponticum to chromosome 7D of wheat (Triticum aestivum) and confers resistance to both Fusarium head blight (FHB) and Fusarium crown rot (FCR). However, Fhb7 is tightly linked to the PSY-E2 gene, which causes yellow flour, limiting its application in breeding. To break this linkage, marker K-PSY was developed for tagging PSY-E2 and used with Fhb7 markers to identify recombination between the two genes. Screening 21,000 BC1F2 backcross progeny (Chinese Spring ph1bph1b*2/SDAU 2028) revealed two Fhb7' wheat-Tp7el2L lines, Shannong 2-16 and Shannong 16-1, that carry a desired truncated Fhb7' translocation segment without PSY-E2. The two lines show levels of resistance to FHB and FCR similar to those of the original translocation line SDAU 2028, but have white flour. To facilitate Fhb7 use in wheat breeding, STS markers were developed and used to isolate Fhb7 on a truncated Tp7el2 translocation segment. Near-isogenic lines carrying the Fhb7' segment were generated in the backgrounds of three commercial cultivars, and Fhb7' lines showed increased FHB and FCR resistance without yield penalty. The breakage of the tight linkage between Fhb7 and PSY-E2 via homoeologous recombination provides genetic resources for improvement of wheat resistance to FHB and FCR and permit the large-scale deployment of Fhb7 in breeding using marker-assisted selection. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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