Preparation of soybean meal peptide for chelation with copper/zinc using Aspergillus oryzae in solid-state fermentation

文献类型： 外文期刊

第一作者： Li, Congmiao

作者： Li, Congmiao;Cao, Leipeng;Liu, Yuhuan;Fan, Ruijuan;Wang, Yunpu;Liu, Tongying;Huang, Zhenghua

作者机构：

关键词： Soybean meal peptide; Solid-state fermentation; Aspergillus oryzae; Chelate; Copper; Zinc

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.2； 五年影响因子：5.4 ）

ISSN： 2212-4292

年卷期： 2023 年 53 卷

页码：

收录情况： SCI

摘要： This study investigated the optimal process of solid-state fermentation (SSF) using soybean meal (SBM) as substrate by Aspergillus oryzae and proteolysis of SBM to prepare SBM-derived peptide (SBMP) for chelating with Cu(II)/Zn(II). During the SSF of SBM, the alkaline protease activity of A. oryzae was higher than that of two other fungi, reaching maximum activity (574 U/g) at the fermentation time of 86 h, 43% inoculation ratio, and liquid-substrate (I/S) ratio of 0.8. The SBMP yield reached a maximum value at 55 degrees C for 6 h hydrolysis according to the analysis of the degree of hydrolysis (DH) and total Kjeldahl nitrogen (TKN) content in hydrolysates. After ultrafiltration, the SBMP of 1-5 kDa had the best ability to chelate with Cu(II) and Zn(II). Moreover, the SBMP displayed a higher capacity to chelate with Zn(II) (222.7 mg/g) than Cu(II) (68.3 mg/g) under optimal conditions. Aspartic acid, glutamic acid, lysine, arginine and histidine in SBMP played a critical role at the chelating Cu(II)/Zn(II). UV and FTIR spectrum analysis revealed that the -COOH and -NH2 in SBMP were the main bioactive groups for chelating with Cu(II)/Zn(II). SEM showed that the surface of SBMP-Cu(II)/Zn(II) chelate was rough and formed spherical aggregates. These results indicated that combining SSF and proteolysis exhibited great potential for preparing SBMP-metal chelates for developing organometallic supplements.

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