Alterations in Whey Protein Abundance Correlated with the Somatic Cell Count Identified via Label-Free and Selected Reaction Monitoring Proteomic Approaches

文献类型： 外文期刊

第一作者： Li, Jing

作者： Li, Jing;Chen, Kaixu;Zang, Changjiang;Zhao, Xiaowei;Cheng, Zhiqiang;Li, Xiaobin;Wang, Caidie;Chen, Yong;Yang, Kailun;Zhao, Xiaowei

作者机构：

关键词： somatic cell count; milk; whey protein; proteomics

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2025 年 15 卷 5 期

页码：

收录情况： SCI

摘要： The somatic cell count (SCC) is widely used to assess milk quality and diagnose intramammary infections. Several whey proteins have been shown to correlate significantly with SCC and are considered potential indicators of udder health. However, the relationship between milk whey proteins and SCC has not been fully elucidated. In this study, milk samples were grouped into five categories based on SCC levels. Subsequently, whey proteins were identified using a label-free proteomics approach, and the differential abundance of proteins was validated through a selected reaction monitoring (SRM) method. The levels of various proteins, including azurocidin 1 and kininogen-2, exhibited an increase, whereas topoisomerase I, tropomyosin-1, and desmin showed a significant decrease depending on the SCCs. Principal component analysis unveiled that these proteins contributed to the developmental alterations in milk proteins. A majority of these differentially abundant proteins were associated with response to stimulus, localization, and defense response. Our results provide fundamental information on the SCC that can be utilized for evaluating milk quality and serve as potential indicators for detecting intramammary infections.

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