siRNA targeting Atp5a1 gene encoding ATPase a, the ligand of Peg fimbriae, reduced Salmonella Enteritidis adhesion

文献类型： 外文期刊

第一作者： Zhang, Di

作者： Zhang, Di;Jiang, Yi;Zhuang, Linlin;Dou, Xinhong;Xu, Bu;Gong, Jiansen;Dong, Yongyi;Wu, Kun;Fu, Lixia;Wang, Chengming

作者机构：

关键词： Salmonella Enteritidis; Peg fimbriae; ligand blot; ATPase & alpha; siRNA; adhesion

期刊名称：AVIAN PATHOLOGY （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN： 0307-9457

年卷期： 2023 年 52 卷 6 期

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收录情况： SCI

摘要： Salmonella enterica serovar Enteritidis (S. Enteritidis) is a zoonotic pathogen that can infect both humans and animals. Among the 13 types of fimbrial operons in S. Enteritidis, the highly conserved Peg fimbriae play a crucial role in the adhesion and invasion of S. Enteritidis into host cells but are not well studied. In this study, we identified the ATP synthase subunit alpha (ATPase a) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase a to the purified adhesion protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase a gene Atp5a1 in Leghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase a in the adhesion process.

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