The Pseudotyped Replication-Deficient VSV with Spike from PEDV Induces Neutralizing Antibody Against PEDV

文献类型： 外文期刊

第一作者： Yi, Jingxuan

作者： Yi, Jingxuan;Zhang, Kang;Wei, Zuzhang;Yi, Jingxuan;Luo, Huaye;Zhang, Kang;Lv, Lilei;Li, Siqi;Jiang, Yifeng;Zhou, Yanjun;Liu, Changlong;Jiang, Yifeng;Zhou, Yanjun;Liu, Changlong

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关键词： porcine epidemic diarrhea virus; spike protein; vesicular stomatitis virus; pseudovirus; vaccine

期刊名称：VACCINES （ 影响因子：3.4； 五年影响因子：3.7 ）

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年卷期： 2025 年 13 卷 3 期

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收录情况： SCI

摘要： Background: Porcine epidemic diarrhea virus (PEDV) is a significant pathogen in swine, causing substantial economic losses worldwide. Despite the availability of existing vaccines, there is a critical need for novel vaccine platforms that ensure robust protection while maintaining safety. Methods: A recombinant replication-deficient vesicular stomatitis virus (VSV) vaccine, rVSV triangle G-PEDV-S, was developed by pseudotyping the virus with the spike (S) protein from PEDV. To achieve high-titer pseudotyped rVSV particles, a stable Huh7 cell line expressing the PEDV S protein (Huh7-PEDV-S) was generated. The infectivity and replication capacity of rVSV triangle G-PEDV-S were evaluated in PEDV-susceptible cell lines and Huh7-PEDV-S cells. The vaccine's immunogenicity and safety were assessed in BALB/c mice vaccinated intramuscularly with rVSV triangle G-PEDV-S. Results: The pseudotyped rVSV triangle G-PEDV-S demonstrated infectivity in PEDV-susceptible cell lines and robust replication in Huh7-PEDV-S cells, while remaining replication-deficient in non-complementary cells. In vaccinated BALB/c mice, the vaccine elicited a strong humoral immune response, characterized by high levels of PEDV S1-specific IgG and neutralizing antibodies. No adverse effects, including weight loss or behavioral changes, were observed in the vaccinated mice, confirming the vaccine's safety. Conclusions: The rVSV triangle G-PEDV-S vaccine represents a promising platform for controlling PEDV outbreaks. Its replication-deficient design and pseudotyping methodology ensure safety and adaptability to emerging PEDV variants. These findings highlight the potential of rVSV triangle G-PEDV-S as a safe and effective solution to the ongoing challenges posed by PEDV.

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