Genome-Wide Identification of the WUSCHEL-Related Homeobox (WOX) Gene Family in Barley Reveals the Potential Role of HvWOX8 in Salt Tolerance

文献类型： 外文期刊

第一作者： Zhang, Wenqi

作者： Zhang, Wenqi;Huang, Linli;Zhou, Longhua;Zong, Yingjie;Gao, Runhong;Li, Yingbo;Liu, Chenghong;Zhang, Wenqi;Huang, Linli;Zhou, Longhua;Zong, Yingjie;Gao, Runhong;Li, Yingbo;Liu, Chenghong

作者机构：

关键词： Hordeum vulgare; WUSCHEL-related homeobox; plant regeneration; expression pattern; salt tolerance

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 5 期

页码：

收录情况： SCI

摘要： The WUSCHEL-related homeobox (WOX) belongs to a plant-specific transcription factor gene family that plays crucial roles in plant growth and development. Barley ranks as the fourth global cereal crop and is recognized as a model crop for the study of cereal genetics. However, genome-wide characterization, functional validation, and stress-related studies of the WOX gene family in barley remain limited, hindering efforts to leverage their potential for improving salt tolerance and regeneration efficiency in breeding programs. In this study, we identified 12 HvWOX genes assigned from chromosome 1 to chromosome 5. Phylogenetic analysis revealed that these HvWOX genes can be classified into three clades (WUS, ancient, and intermediate). Gene structure analysis revealed that the exon numbers of HvWOX genes varied in the WUS and intermediate clades but were highly conserved in the ancient clade. Tissue-specific analysis revealed that the most common HvWOX genes were highly expressed in reproductive tissues such as anthers or ovaries. Cis-element analysis suggested that there were multiple stress- and hormone-responsive elements in the HvWOX gene promoters. In addition, overexpression of HvWOX8 in Arabidopsis significantly enhanced root elongation under salt stress (50-100 mM NaCl), suggesting its direct role in salt tolerance. Transcriptomic analysis further revealed that HvWOX8 modulates hormone signaling and electron transfer pathways during ATP synthesis under stress conditions. In conclusion, our results provided a comprehensive understanding of the gene characteristics, expression patterns, and potential roles of barley WOX genes.

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