Extraction, identification and anti-photoaging activity evaluation of collagen peptides from silver carp (Hypophthalmichthys molitrix) skin

文献类型： 外文期刊

第一作者： Huang, Jia-jun

作者： Huang, Jia-jun;Li, Hai-lan;Xiong, Guang-quan;Liao, Tao;Zu, Xiao-yan;Huang, Jia-jun;Cai, Jun;Xiong, Guang-quan;Liao, Tao;Zu, Xiao-yan;Zu, Xiao-yan;Cai, Jun

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关键词： Sliver carp skin collagen peptide; Anti-photoaging; Peptide sequence; Fibroblasts; Matrix metalloproteinase-1

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）

ISSN： 0023-6438

年卷期： 2023 年 173 卷

页码：

收录情况： SCI

摘要： Biologically active peptides are particularly important for anti-photoaging studies. In this study, silver carp (Hypophthalmichthys molitrix) skin collagen polypeptide (SCSCP) with collagenase inhibition activity was obtained by optimizing the enzymatic hydrolysis process. Fourier-transform infrared spectroscopy, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and liquid-liquid mass spectrometry were used to analyze the secondary structure, molecular weight distribution and characteristics of peptides, respectively. The effects of SCSCP on the proliferation of mouse fibroblasts (L929) and secretion of matrix metalloproteinase-1 (MMP-1) after UVB irradiation were also detected. Process-optimized SCSCP inhibited collagenase activity up to 54.47%. In SCSCP, alpha-helical and beta-folded structures accounted for 17.70% and 22.65%, respectively. The relative molecular weights of SCSCP were more widely distributed below 1600 Da, with higher response values at 665.93 and 980.95 Da. The molecular weights of the characteristic peptides were 358.68-863.89 Da, and the peptide sequences with anti-photoaging activity were GPPGPPGTPGPQ, SGLPGPIGPPGPR, and GLPGPIGPPGPR. SCSCP at 10 mg/mL concentration had high DPPH and hydroxyl radical scavenging activity, while 0.1 mg/mL SCSCP significantly restored UVB-induced L929 cell viability and inhibited MMP-1 secretion in L929 cells after UVB irradiation. These results indicate that SCSCP achieves anti-photoaging effects by scavenging DPPH and hydroxyl radicals and inhibiting MMP-1 secretion.

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