PbrCalS5, a callose synthase protein, is involved in pollen tube growth in Pyrus bretschneideri

文献类型： 外文期刊

第一作者： Cao, Peng

作者： Cao, Peng;Tang, Chao;Wu, Xiao;Qian, Ming;Lv, Shouzheng;Gao, Hongru;Qiao, Xin;Chen, Guodong;Wang, Peng;Zhang, Shaoling;Wu, Juyou;Wu, Juyou;Chen, Guodong;Tang, Chao;Wu, Juyou

作者机构：

关键词： CalS; Evolution; Pear; Callose; Pollen tube

期刊名称：PLANTA （ 影响因子：4.54； 五年影响因子：4.689 ）

ISSN： 0032-0935

年卷期： 2022 年 256 卷 2 期

页码：

收录情况： SCI

摘要： Main conclusion Identification of CalS genes in seven Rosaceae species and functional characterization of PbrCalS5 in pear pollen tube growth by regulating callose deposition. Callose exists widely in angiosperms and has significant functions in a range of developmental processes. Callose is synthesized by callose synthase (CalS). However, the members of the callose synthase gene family and their evolutionary profiles, along with their biological functions, in species of the Rosaceae remain unknown. In this study, a total of 69 members of the CalS gene family in seven Rosaceae species (Fragaria vesca, Malus x domestica, Prunus avium, Pyrus bretschneideri, Prunus mume, Prunus persica and Rubus occidentalis) were identified and divided into six clades. Different types of gene duplication events contributed to the expansions of the CalS gene family in the seven species, with purifying selection playing a key role in the evolution of the CalS genes. Tissue-specific expression patterns analysis revealed that PbrCalS5 was highly expressed in the pear pollen tube and was selected for further functional analysis. Subcellular localization indicated that PbrCalS5 was localized in the plasma membrane and cell wall. Antisense oligodeoxynucleotide (AS-ODN) assays resulted in the inhibition of PbrCalS5 expression, leading to the decreased callose deposition in the pollen tube wall and subsequent inhibition of pear pollen tube growth. These results provide the theoretical basis for exploring the functional roles of CalS genes in pear pollen tube growth.

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