Structural Basis and Recognition Mechanism of Host-Derived Volatiles by Olfactory Proteins in the Agricultural Pest Bactrocera correcta (Diptera: Tephritidae)

文献类型： 外文期刊

第一作者： Guo, Tengda

作者： Guo, Tengda;Zhang, Yuan;Huang, Yumeng;Li, Zhihong;Qin, Yujia;Zhang, Yongjun

作者机构：

关键词： Bactrocera correcta; VOCs; olfactoryproteins; protein structure; molecular dynamicssimulation; recognition mechanism

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 21 期

页码：

收录情况： SCI

摘要： Host-derived volatiles play a critical role in mediating plant-insect interactions. Bactrocera correcta is a destructive pest of fruit crops. In this study, we investigated the recognition mechanisms of three key volatiles-beta-caryophyllene, ethyl decanoate, and hexyl hexanoate-derived from the host fruits of B. correcta. Using transcriptomic analysis, fluorescence binding assays, molecular docking, and molecular dynamics simulations, we identified BcorOBP19d-2 as a key odorant-binding protein that binds multiple volatiles and facilitates their stabilization and transport. Odorant receptors (BcorOR7a-13, BcorOR74a-3, and BcorOR7a-3) selectively recognize these volatiles, with hydrophobic interactions as the primary driving force for binding. beta-Caryophyllene exhibited the highest binding specificity with BcorOR7a-13, ethyl decanoate demonstrated the strongest binding affinity with BcorOR74a-3, and hexyl hexanoate showed moderate stability with BcorOR7a-3. These findings provide structural insights into volatile recognition in polyphagous insects and offer a basis for developing attractants or repellents for pest management.

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