Host-induced silencing of a nematode chitin synthase gene decreases abundance of rhizosphere fungal community while enhancing Heterodera glycines resistance of soybean

文献类型： 外文期刊

第一作者： Tian, Shuan

作者： Tian, Shuan;Shi, Xue;Kang, Houxiang;Huang, Wenkun;Peng, Huan;Peng, Deliang;Liu, Shiming;Kong, Lingan;Qu, Baoyuan;Wang, Jiajun

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关键词： abundance; chitin synthase; diversity; host-induced silencing of gene; rhizosphere microbial community; SCN resistance; soybean; transgenic soybean H57

期刊名称：CROP & PASTURE SCIENCE （ 影响因子：2.249； 五年影响因子：2.541 ）

ISSN： 1836-0947

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收录情况： SCI

摘要： Context. A transgenic variety of soybean (Glycine max (L.) Merr.), H57, has been developed from wild-type variety Jack, with host-induced gene silencing of a chitin synthase gene (CHS) in soybean cyst nematode (SCN, Heterodera glycines Ichinohe), a devastating pathogen in soybean. H57 needs to be characterised for suitability to manage SCN, especially because rhizosphere microbial communities may be sensitive to genetically modified crops. Aims. We aimed to evaluate the SCN resistance of H57 at the T-7 generation, and analyse the impact on the rhizosphere microbial community of planting H57 into SCN-infected soil. Methods. Infection with SCN was assessed at 60 days after planting of H57 and Jack into SCN-infected soil by examining recovered cysts from rhizosphere soil and comparing with an infected bulk soil control. For analysis of rhizosphere microbial communities (bacterial and fungal), 16S and ITS amplicons were identified by high-throughput sequencing, and bioinformatic analysis was used to define operational taxonomic units. Alpha diversity, using five indexes, and relative abundance were determined. Key results. Soybean H57 showed significantly enhanced and heritable resistance to SCN compared with Jack. The diversity and richness (abundance) of the bacterial community of H57 and Jack were significantly and similarly increased relative to the bulk soil. The fungal community of H57 had considerably lower abundance than both other treatments, and lower diversity than the bulk soil. The relative abundance of only two bacterial phyla (Acidobacteria and Actinobacteria) and one fungal phylum (Glomeromycota), and three bacterial genera (Candidatus_Solibacter, Candidatus_Udaeobacter and Bryobacter) and one fungal genus (Aspergillus), differed significantly between rhizosphere soils of H57 and Jack. Conclusions. Host-induced gene silencing of SCN-CHS substantially and heritably enhanced SCN resistance in soybean, did not significantly alter the rhizosphere bacterial community, but greatly suppressed the abundance of the rhizosphere fungal community, which was likely associated with boosted SCN resistance.

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[1]Host-induced silencing of a nematode chitin synthase gene decreases abundance of rhizosphere fungal community while enhancing Heterodera glycines resistance of soybean. Tian, Shuan,Shi, Xue,Kang, Houxiang,Huang, Wenkun,Peng, Huan,Peng, Deliang,Liu, Shiming,Kong, Lingan,Qu, Baoyuan,Wang, Jiajun.

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