Evaluation of Dry Ice for Short-Term Storage and Transportation of Frozen Boar Semen
文献类型: 外文期刊
第一作者: He, Mengqian
作者: He, Mengqian;Sun, Lingwei;Xu, Jiehuan;Wu, Caifeng;Zhang, Shushan;Gao, Jun;Zhang, Defu;Dai, Jianjun;He, Mengqian;Sun, Lingwei;Xu, Jiehuan;Wu, Caifeng;Zhang, Shushan;Gao, Jun;Zhang, Defu;Dai, Jianjun;He, Mengqian;Sun, Lingwei;Xu, Jiehuan;Wu, Caifeng;Zhang, Shushan;Zhang, Defu;Han, Xuejun;Dai, Jianjun;Gan, Yeqing;Bian, Yi;Wei, Jinliang;Zhang, Weijian;Zhang, Wengang
作者机构:
关键词: semen cryopreservation; dry ice; semen transportation; boar
期刊名称:ANIMALS ( 影响因子:3.0; 五年影响因子:3.2 )
ISSN: 2076-2615
年卷期: 2024 年 14 卷 10 期
页码:
收录情况: SCI
摘要: To address the safety problems posed by the transportation of boar semen using LN, this study was conducted on the short-term storage of frozen boar semen in dry ice (-79 degrees C). Boar semen frozen in LN was transferred to dry ice, kept for 1 day, 3 days, 5 days, 7 days, or 8 days, and then moved back to LN. The quality of frozen semen stored in LN or dry ice was determined to evaluate the feasibility of short-distance transportation with dry ice. The results showed that 60 degrees C for 8 s was the best condition for thawing frozen semen stored in dry ice. No significant differences in spermatozoa motility, plasma membrane integrity, or acrosome integrity were observed in semen after short-term storage in dry ice compared to LN (p > 0.05). There were no significant changes in antioxidant properties between storage groups either (p > 0.05). In conclusion, dry ice could be used as a cold source for the short-term transportation of frozen boar semen for at least 7 days, without affecting sperm motility, morphological integrity, or antioxidant indices.
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