Therapeutic function of iPSCs-derived primitive neuroepithelial cells in a rat model of Parkinson's disease

文献类型： 外文期刊

第一作者： Guo, Yu

作者： Guo, Yu;Zhu, Huan;Sun, Tingting;Wang, Yuanyuan;Huang, Yuqi;Ma, Caiyun;Wang, Chunjing;Liu, Changqing;Guan, Yuhan;Ma, Caiyun;Guan, Weijun;Emery, Rik;Liu, Changqing

作者机构：

关键词： Neuroepithelial cells; Parkinson's disease; Rat induced pluripotent stem cells; Cell transplantation

期刊名称：NEUROCHEMISTRY INTERNATIONAL （ 影响因子：4.297； 五年影响因子：4.567 ）

ISSN： 0197-0186

年卷期： 2022 年 155 卷

页码：

收录情况： SCI

摘要： Induced pluripotent stem cells (iPSCs) are a promising unlimited source for cell replacement therapy of neurodegenerative disorders, including Parkinson's disease (PD). In the present study, rat iPSCs-derived primitive neuroepithelial cells (RiPSCs-iNECs) were successfully induced from rat iPSCs (RiPSCs) following two major developmental stages, and could generate neurospheres and differentiated into both neurons and astrocytes in vitro. Then, the RiPSCs-iNECs-GFP(+) were unilaterally transplanted into the right substantia nigra (SN) of 6-hydroxydopamine-lesioned rat models of PD. The results demonstrated that the grafted RiPSCs-iNECs could survive in parkinsonian rat brain for at least 150 days, and many of them differentiated into tyrosine hydroxylase (TH)-positive cells. Furthermore, the PD model rats grafted with RiPSCs-iNECs exhibited a significant functional recovery from their parkinsonian behavioral defects. Histological studies showed that RiPSCs-iNECs could differentiate into multiple types of neurons including dopaminergic neurons, GFAP, Pax6, FoxA2 and DAT-positive cells, and induced dopaminergic neurons extended dense neurites into the host striatum. Thus, iPSCs derived primitive neuroepithelial cells could be an attractive candidate as a source of donor material for the treatment of PD, but the molecular mechanism needs further clarification.

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