Genistein Alleviates Intestinal Oxidative Stress by Activating the Nrf2 Signaling Pathway in IPEC-J2 Cells

文献类型： 外文期刊

第一作者： Li, Yanpin

作者： Li, Yanpin;Cai, Long;Bi, Qingyue;Sun, Wenjuan;Pi, Yu;Jiang, Xianren;Li, Xilong;Bi, Qingyue

作者机构：

关键词： genistein; antioxidation; Nrf2 signaling pathway; intestinal epithelial cells; piglets

期刊名称：VETERINARY SCIENCES （ 影响因子：2.4； ）

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年卷期： 2024 年 11 卷 4 期

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收录情况： SCI

摘要： Simple Summary: Interest in using natural feed additives in animal diets to improve health and productivity has grown significantly over the past few decades. Genistein is an aglycone form of soybean isoflavones with higher antioxidant activity. However, whether genistein can alleviate oxidative stress in pig intestines and the precise mechanism behind this effect remains to be elucidated. In the present research, the hydrogen peroxide-stimulated IPEC-J2 cells oxidative stress model was employed to explore the antioxidant capacity of genistein and potential mechanisms. The results showed that genistein could exert a protective effect against hydrogen peroxide-stimulated oxidative stress by activating the Nrf2 signaling pathway in IPEC-J2 cells. These results could provide a novel nutritional intervention strategy to enhance the intestinal health of piglets under oxidative stress. In the weaning period, piglets often face oxidative stress, which will cause increased diarrhea and mortality. Genistein, a flavonoid, which is extracted from leguminous plants, possesses anti-inflammatory and antioxidative bioactivities. However, little is known about whether genistein could attenuate the oxidative stress that occurs in porcine intestinal epithelial cells (IPEC-J2). Herein, this experiment was carried out to investigate the protective effects of genistein in the IPEC-J2 cells oxidative stress model. Our results disclosed that H2O2 stimulation brought about a significant diminution in catalase (CAT) activity and cell viability, as well as an increase in the levels of reactive oxygen species (ROS) in IPEC-J2 cells (p < 0.05), whereas pretreating cells with genistein before H2O2 exposure helped to alleviate the reduction in CAT activity and cell viability (p < 0.05) and the raise in the levels of ROS (p = 0.061) caused by H2O2. Furthermore, H2O2 stimulation of IPEC-J2 cells remarkably suppressed gene level Nrf2 and CAT expression, in addition to protein level Nrf2 expression, but pretreating cells with genistein reversed this change (p < 0.05). Moreover, genistein pretreatment prevented the downregulation of occludin expression at the gene and protein level, and ZO-1 expression at gene level (p < 0.05). In summary, our findings indicate that genistein possesses an antioxidant capacity in IPEC-J2 cells which is effective against oxidative stress; the potential mechanism may involve the Nrf2 signaling pathway. Our findings could offer a novel nutritional intervention strategy to enhance the intestinal health of piglets during the weaning process.

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