Knockdown of 60S ribosomal protein L14-2 reveals their potential regulatory roles to enhance drought and salt tolerance in cotton

文献类型： 外文期刊

第一作者： Shiraku, Margaret Linyerera

作者： Shiraku, Margaret Linyerera;Magwanga, Richard Odongo;Cai Xiaoyan;Kirungu, Joy Nyangasi;Xu Yanchao;Mehari, Teame Gereziher;Hou Yuqing;Wang Yuhong;Wang Kunbo;Zhou Zhongli;Liu Fang;Magwanga, Richard Odongo;Peng Renhai;Liu Fang

作者机构：

关键词： Abiotic stress; Cotton; Ribosomal protein large; Transcription factor; Virus-induced gene silencing

期刊名称：JOURNAL OF COTTON RESEARCH （ 影响因子：2.6； 五年影响因子：2.8 ）

ISSN： 2096-5044

年卷期： 2021 年 4 卷 1 期

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收录情况： SCI

摘要： Background Cotton is a valuable economic crop and the main significant source of natural fiber for textile industries globally. The effects of drought and salt stress pose a challenge to strong fiber and large-scale production due to the ever-changing climatic conditions. However, plants have evolved a number of survival strategies, among them is the induction of various stress-responsive genes such as the ribosomal protein large (RPL) gene. The RPL gene families encode critical proteins, which alleviate the effects of drought and salt stress in plants. In this study, comprehensive and functional analysis of the cotton RPL genes was carried out under drought and salt stresses. Results Based on the genome-wide evaluation, 26, 8, and 5 proteins containing the RPL14B domain were identified in Gossypium hirsutum, G. raimondii, and G. arboreum, respectively. Furthermore, through bioinformatics analysis, key cis-regulatory elements related to RPL14B genes were discovered. The Myb binding sites (MBS), abscisic acid-responsive element (ABRE), CAAT-box, TATA box, TGACG-motif, and CGTCA-motif responsive to methyl jasmonate, as well as the TCA-motif responsive to salicylic acid, were identified. Expression analysis revealed a key gene, Gh_D01G0234 (RPL14B), with significantly higher induction levels was further evaluated through a reverse genetic approach. The knockdown of Gh_D01G0234 (RPL14B) significantly affected the performance of cotton seedlings under drought/salt stress conditions, as evidenced by a substantial reduction in various morphological and physiological traits. Moreover, the level of the antioxidant enzyme was significantly reduced in VIGS-plants, while oxidant enzyme levels increased significantly, as demonstrated by the higher malondialdehyde concentration level. Conclusion The results revealed the potential role of the RPL14B gene in promoting the induction of antioxidant enzymes, which are key in oxidizing the various oxidants. The key pathways need to be investigated and even as we exploit these genes in the developing of more stress-resilient cotton germplasms.

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