Evaluation of protective immune responses induced by DNA vaccines encoding Echinococcus granulosus EgM123 protein in Beagle dogs

文献类型： 外文期刊

第一作者： Wang, Ning

作者： Wang, Ning;Xian, Jinwen;Zhao, Pengpeng;Zhao, Wenqing;Pu, Na;Jia, Xinyue;Zhang, Yanyan;Bo, Xinwen;Wang, Zhengrong;Wang, Ning;Xian, Jinwen;Zhao, Pengpeng;Zhao, Wenqing;Pu, Na;Jia, Xinyue;Bo, Xinwen;Wang, Zhengrong;Wang, Ning;Xian, Jinwen;Zhao, Pengpeng;Pu, Na;Jia, Xinyue;Bo, Xinwen;Zhao, Wenqing

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关键词： Echinococcus granulosus; EgM123; DNA vaccine; definitive-host; worm burden

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：2.9； 五年影响因子：3.3 ）

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年卷期： 2024 年 11 卷

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收录情况： SCI

摘要： Introduction: Echinococcus granulosus, known as cystic echinococcosis, is a prominent zoonotic parasitic disease of significant global concern. The definitive hosts serves as the primary reservoir for the transmission of echinococcosis, as well as a main factor in the prevention and control of the disease. Unfortunately, there is currently no commercially available vaccine for these hosts. Nevertheless, DNA vaccines show potential as a feasible strategy for the control and management of parasitic diseases. Methods: In this study, the EgM123 antigen was selected for its well-documented immunogenic properties to develop a DNA vaccine aimed at combating E. granulosus infection in canines. Results: The results showed a marked increase in IgG levels in the group vaccinated with pVAX1-EgM123 DNA compared to the PBS group. Additionally, the cytokines IL-1, IFN-gamma, IL-4, and IL-6 were significantly upregulated in the pVAX1-EgM123 DNA vaccine group. Furthermore, in comparison to the PBS control group, the EgM123 DNA vaccine group exhibited a notable 87.85% reduction in worm burden and a 65.00% inhibition in segment development. Discussion: These findings indicate that the pVAX1-EgM123 DNA vaccine shows promising immunogenicity, successfully eliciting a targeted immune response in canines. Moreover, it significantly diminishes the worm burden and hinders the progression of tapeworms in the pVAX1-EgM123 DNA vaccine group. These findings suggest that the pVAX1-EgM123 DNA vaccine holds promise as a potential candidate vaccine for combating E. granulosus infection in dogs

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