Microcystin-LR induces apoptosis in Juvenile Eriocheir sinensis via the mitochondrial pathway

文献类型： 外文期刊

第一作者： Zhang, Yuning

作者： Zhang, Yuning;Xu, Gangchun;Xu, Pao;Gao, Jiancao;Nie, Zhijuan;Zhu, Haojun;Du, Jinliang;Cao, Liping;Shao, Nailin;Sun, Yi;Su, Shengyan;Xu, Gangchun;Xu, Pao

作者机构：

关键词： Microcystin-LR; Eriocheir sinensis; Hepatopancreas injury; Apoptosis; AP-1

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：7.129； 五年影响因子：7.284 ）

ISSN： 0147-6513

年卷期： 2022 年 238 卷

页码：

收录情况： SCI

摘要： Microcystin-LR (MC-LR), the toxic substance of cyanobacteria secondary metabolism, widely exists in water environments and poses great risks to living organisms. Some toxicological assessments of MC-LR have performed at physiological and biochemical levels. However, plenty of blanks about the potential mechanism in aquatic crustacean remains. In this study, we firstly assessed the exposure toxicity of MC-LR to juvenile E. sinensis and clarified that the 96 h LD50 of MC-LR was 73.23 mu g/kg. Then, hepatopancreas transcriptome profiles of MCLR stressed crabs were constructed at 6 h post-injection and 37 differential expressed genes (DEGs) were identified. These DEGs were enriched in cytoskeleton, peroxisome and apoptosis pathways. To further reveal the toxicity of MC-LR, oxidative stress parameters (SOD, CAT, GSH-px and MDA), apoptosis genes (caspase 3, bcl-2 and bax) and apoptotic cells were detected. Significant accumulated MDA and rise-fall enzyme activities verified the oxidative stress caused by MC-LR. It is noteworthy that quantitative real-time PCR and TUNEL assay indicated that MC-LR stress-induced apoptosis via the mitochondrial pathway. Interestingly, activator protein-1 may play a crucial role in mediating the hepatotoxicity of MC-LR by regulating apoptosis and oxidative stress. Taken together, our study investigated the toxic effects and the potential molecular mechanisms of MC-LR on juvenile E. sinensis. It provided useful data for exploring the toxicity of MC-LR to aquatic crustaceans at molecular levels.

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