Potential Misidentification of Natural Isomers and Mass-Analogs of Modified Nucleosides by Liquid Chromatography-Triple Quadrupole Mass Spectrometry
文献类型： 外文期刊
第一作者： Lin, Xiuying
作者： Lin, Xiuying;Qin, Yichao;Fu, Pengcheng;Lin, Huan;Lin, Xiuying;Zhang, Qianhui;Zhong, Qisheng;Lv, Daizhu;Wu, Xiaopeng
作者机构：
关键词： nucleoside misidentification; RNA modification; epitranscriptomics; LC-MS; MS; tRNA; HILIC
期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）
ISSN：
年卷期： 2022 年 13 卷 5 期
页码：
收录情况： SCI
摘要： Triple quadrupole mass spectrometry coupled to liquid chromatography (LC-TQ-MS) can detect and quantify modified nucleosides present in various types of RNA, and is being used increasingly in epitranscriptomics. However, due to the low resolution of TQ-MS and the structural complexity of the many naturally modified nucleosides identified to date (>160), the discrimination of isomers and mass-analogs can be problematic and is often overlooked. This study analyzes 17 nucleoside standards by LC-TQ-MS with separation on three different analytical columns and discusses, with examples, three major causes of analyte misidentification: structural isomers, mass-analogs, and isotopic crosstalk. It is hoped that this overview and practical examples will help to strengthen the accuracy of the identification of modified nucleosides by LC-TQ-MS.
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