Potential Misidentification of Natural Isomers and Mass-Analogs of Modified Nucleosides by Liquid Chromatography-Triple Quadrupole Mass Spectrometry

文献类型： 外文期刊

第一作者： Lin, Xiuying

作者： Lin, Xiuying;Qin, Yichao;Fu, Pengcheng;Lin, Huan;Lin, Xiuying;Zhang, Qianhui;Zhong, Qisheng;Lv, Daizhu;Wu, Xiaopeng

作者机构：

关键词： nucleoside misidentification; RNA modification; epitranscriptomics; LC-MS; MS; tRNA; HILIC

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 5 期

页码：

收录情况： SCI

摘要： Triple quadrupole mass spectrometry coupled to liquid chromatography (LC-TQ-MS) can detect and quantify modified nucleosides present in various types of RNA, and is being used increasingly in epitranscriptomics. However, due to the low resolution of TQ-MS and the structural complexity of the many naturally modified nucleosides identified to date (>160), the discrimination of isomers and mass-analogs can be problematic and is often overlooked. This study analyzes 17 nucleoside standards by LC-TQ-MS with separation on three different analytical columns and discusses, with examples, three major causes of analyte misidentification: structural isomers, mass-analogs, and isotopic crosstalk. It is hoped that this overview and practical examples will help to strengthen the accuracy of the identification of modified nucleosides by LC-TQ-MS.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序