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Creation of poxvirus expressing foot-and-mouth and peste des petits ruminant disease virus proteins

文献类型：外文期刊

第一作者： Zhao, Zhixun

作者： Zhao, Zhixun;Huang, Caiyun;Zhu, Xueliang;Qi, Zheng;Cao, Yimei;Li, Pinghua;Bao, Huifang;Sun, Pu;Bai, Xingwen;Fu, Yuanfang;Li, Kun;Zhang, Jing;Ma, Xueqing;Wang, Jian;Yuan, Hong;Li, Dong;Liu, Zaixin;Zhang, Qiang;Lu, Zengjun

作者机构：

关键词： Foot-and-mouth disease virus; Peste des petits ruminant disease virus; Vaccinia virus Ankara strain; Recombinant live vector vaccine

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （影响因子：5.0；五年影响因子：5.2 ）

ISSN： 0175-7598

年卷期： 2023 年 107 卷 2-3 期

页码：

收录情况： SCI

摘要： Objective Foot-and-mouth disease (FMD) and Peste des petits ruminant disease (PPR) are acute and severe infectious diseases of sheep and are listed as animal diseases for compulsory immunization. However, there is no dual vaccine to prevent these two diseases. The Modified Vaccinia virus Ankara strain (MVA) has been widely used in the construction of recombinant live vector vaccine because of its large capacity of foreign gene, wide host range, high safety, and immunogenicity. In this study, MVA-GFP recombinant virus skeleton was used to construct dual live vector vaccines against FMD and PPR. Methods The recombinant plasmid pUC57-FMDV P1-2A3CPPRV FH was synthesized and transfected into MVA-GFP infected CEF cells for homologous recombination. Results The results showed that a recombinant virus without fluorescent labeling was obtained after multiple rounds of plaque screening. The recombinant virus successfully expressed the target proteins, and the empty capsid of FMDV could be observed by transmission electron microscope (TME), and the expression levels of foreign proteins (VP1 and VP3) detected by ELISA were like those detected in FMDV-infected cells. This study laid the foundation for the successful construction of a live vector vaccine against FMD and PPR.

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