Cu/Zn superoxide dismutase (VdSOD1) mediates reactive oxygen species detoxification and modulates virulence in Verticillium dahliae

文献类型： 外文期刊

第一作者： Tian, Li

作者： Tian, Li;Huang, Caimin;Qiu, Nianwei;Li, Junjiao;Zhang, Dandan;Song, Jian;Wang, Dan;Chen, Jieyin;Dai, Xiaofeng;Xu, Yan;Yang, Xingyong;Xu, Yan;Yang, Xingyong;Short, Dylan P. G.;Inderbitzin, Patrik;Subbarao, Krishna, V

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关键词： reactive oxygen species; ROS detoxification; superoxide dismutase; unconventional secretion; Verticillium dahliae; virulence

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）

ISSN： 1464-6722

年卷期： 2021 年 22 卷 9 期

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收录情况： SCI

摘要： The accumulation of reactive oxygen species (ROS) is a widespread defence mechanism in higher plants against pathogen attack and sometimes is the cause of cell death that facilitates attack by necrotrophic pathogens. Plant pathogens use superoxide dismutase (SOD) to scavenge ROS derived from their own metabolism or generated from host defence. The significance and roles of SODs in the vascular plant pathogen Verticillium dahliae are unclear. Our previous study showed a significant upregulation of Cu/Zn-SOD1 (VdSOD1) in cotton tissues following V. dahliae infection, suggesting that it may play a role in pathogen virulence. Here, we constructed VdSOD1 deletion mutants (Delta SOD1) and investigated its function in scavenging ROS and promoting pathogen virulence. Delta SOD1 had normal growth and conidiation but exhibited significantly higher sensitivity to the intracellular ROS generator menadione. Despite lacking a signal peptide, assays in vitro by western blot and in vivo by confocal microscopy revealed that secretion of VdSOD1 is dependent on the Golgi reassembly stacking protein (VdGRASP). Both menadione-treated Delta SOD1 and cotton roots infected with Delta SOD1 accumulated more O2- and less H2O2 than with the wildtype strain. The absence of a functioning VdSOD1 significantly reduced symptom severity and pathogen colonization in both cotton and Nicotiana benthamiana. VdSOD1 is nonessential for growth or viability of V. dahliae, but is involved in the detoxification of both intracellular ROS and host-generated extracellular ROS, and contributes significantly to virulence in V. dahliae.

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