文献类型： 外文期刊

第一作者： Ying Ziwei

作者： Ying Ziwei;Li Yinkang;Ye Guoling;Xie Xiaoyong;Ying Ziwei;Li Yinkang;Ye Guoling;Xie Xiaoyong;Ying Ziwei;Li Yinkang;Xie Xiaoyong;Ying Ziwei;Xie Xiaoyong;Bao Yuyuan;Zhang Shuhuan;Xu Peng;Zhu Junhua

作者机构：

关键词： Tachypleus tridentatus; diet; reproductive performance; digestive enzyme activity; intestinal flora

期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：1.179； 五年影响因子：1.225 ）

ISSN： 1672-5182

年卷期： 2022 年 21 卷 3 期

页码：

收录情况： SCI

摘要： Effective culture and management of adult tri-spine horseshoe crab, Tachypleus tridentatus can ensure that stock enhancement programs and aquaculture systems are maintained. To explore suitable feed for animals during the breeding season, Pacific oyster (Ostrea gigas) (oyster group; OG) and frozen sharpbelly fish (Hemiculter leucisculus) (frozen fish group; FG) were selected to feed 20 T. tridentatus male and female pairs, respectively. At the end of the experiment, intestinal samples were obtained to measure digestive enzymes activities. The intestinal flora were determined by 16S rDNA sequencing. No eggs were observed in the FG and one T. tridentatus adult died. No animals died in the OG, and 9.7 x 10(4) eggs were obtained. These results show that oysters are more suitable for the development and reproduction of adult T. tridentatus than frozen fish. Additionally, the digestive enzyme activity analysis revealed that animals in the OG exhibited higher protein digestibility than those in the FG, but no significant differences in lipid and carbohydrate uptake were observed between the groups. Furthermore, the intestinal flora analysis showed that operational taxonomic units (OTUs) and the Chao1 index were significantly higher in the OG than in the FG, but no significant difference was observed in the Shannon or Simpson indices between the groups. Our data indicate that the oyster diet improved the intestinal microbial diversity of T. tridentatus. We hypothesize that nutrients, such as oyster-based taurine, proteins, and highly unsaturated fatty acids, improve protease activity in the T. tridentatus digestive tract, alter the intestinal floral structure, and improve the reproductive performance of T. tridentatus.

分类号：