Transcriptomic Responses of Cordyceps militaris to Salt Treatment During Cordycepins Production

文献类型： 外文期刊

第一作者： Lv, Gongbo

作者： Lv, Gongbo;Zhu, Yue;Zeng, Bin;Liu, Xinping;He, Bin;Lv, Gongbo;Zhu, Yue;Zeng, Bin;Liu, Xinping;He, Bin;Cheng, Xiaojie;Cao, Yan;Zeng, Bin

作者机构：

关键词： cordycepin; Cordyceps militaris; salt treatment; molecular mechanism; transcriptome

期刊名称：FRONTIERS IN NUTRITION （ 影响因子：6.59； 五年影响因子：6.873 ）

ISSN： 2296-861X

年卷期： 2021 年 8 卷

页码：

收录情况： SCI

摘要： Cordycepin is a major bioactive compound found in Cordyceps militaris (C. militaris) that exhibits a broad spectrum of biological activities. Hence, it is potentially a bioactive ingredient of pharmaceutical and cosmetic products. However, overexploitation and low productivity of natural C. militaris is a barrier to commercialization, which leads to insufficient supply to meet its existing market demands. In this study, a preliminary study of distinct concentrations of salt treatments toward C. militaris was conducted. Although the growth of C. militaris was inhibited by different salt treatments, the cordycepin production increased significantly accompanied by the increment of salt concentration. Among them, the content of cordycepin in the 7% salt-treated group was five-fold higher than that of the control group. Further transcriptome analysis of samples with four salt concentrations, coupled with Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, several differentially expressed genes (DEGs) were found. Finally, dynamic changes of the expression patterns of four genes involved in the cordycepin biosynthesis pathway were observed by the quantitative real-time PCR. Taken together, our study provides a global transcriptome characterization of the salt treatment adaptation process in C. militaris and facilitates the construction of industrial strains with a high cordycepin production and salt tolerance.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序