Antigenicity and immunogenicity of recombinant proteins comprising African swine fever virus proteins p30 and p54 fused to a cell-penetrating peptide

文献类型： 外文期刊

第一作者： Zhang, Guanglei

作者： Zhang, Guanglei;Liu, Wei;Gao, Zhan;Yang, Sicheng;Zhou, Guangqing;Chang, Yanyan;Ma, Yunyun;Liang, Xiaxia;Shao, Junjun;Chang, Huiyun

作者机构：

关键词： African swine fever virus; Immune response; Cell-penetrating peptide; Recombinant fusion proteins; Vaccine

期刊名称：INTERNATIONAL IMMUNOPHARMACOLOGY （ 影响因子：5.714； 五年影响因子：5.596 ）

ISSN： 1567-5769

年卷期： 2021 年 101 卷

页码：

收录情况： SCI

摘要： African swine fever (ASF) is a highly fatal swine disease threatening the global pig industry. Currently, vaccine is not commercially available for ASF. Hence, it is desirable to develop effective subunit vaccines against ASF. Here, we expressed and purified two recombinant fusion proteins comprising ASFV proteins p30 and p54 fused to a novel cell-penetrating peptide Z12, which were labeled as ZPM (Z12-p30-modified p54) and ZPMT (Z12-p30 modified p54-T cell epitope). Purified recombinant p30 and modified p54 expressed alone or fused served as controls. The transduction capacity of these recombinant proteins was assessed in RAW264.7 cells. Both ZPM and ZPMT exhibited higher transduction efficiency than the other proteins. Subsequently, humoral and cellular immune responses elicited by these proteins were evaluated in mice. ZPMT elicited the highest levels of antigen specific IgG responses, cytokines (interleukin-2, interferon-gamma, and tumor necrosis factor-alpha) and lymphocyte proliferation. Importantly, sera from mice immunized with ZPM or ZPMT neutralized greater than 85% of ASFV in vitro. Our results indicate that ZPMT induces potent neutralizing antibody responses and cellular immunity in mice. Therefore, ZPMT may be a suitable candidate to elicit immune responses in swine, providing valuable information for the development of subunit vaccines against ASF.

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