High-throughput sequencing identification and multiplex RT-PCR detection of pepper viruses

文献类型： 外文期刊

第一作者： Luo, Chun

作者： Luo, Chun;Luo, Yao;Luo, Hao;Han, Ke;Xu, Tengzhi;Lei, Lei;Jia, Meng-ao

作者机构：

关键词： Pepper virus; High-throughput sequencing; Synergistic infection; Multiplex RT-PCR; Virus detection

期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：3.3； 五年影响因子：3.2 ）

ISSN： 0885-5765

年卷期： 2025 年 136 卷

页码：

收录情况： SCI

摘要： Peppers are susceptible to a variety of virus infections. Early and accurate detection of virus species is the prerequisite for the virus disease prevention and management to reduce yield and quality losses. This study utilized small RNA sequencing to investigate the viruses in Guizhou peppers. Through extensive bioinformatics analysis, we identified ten virus species infecting Guizhou peppers and obtained the sequences of the conserved protein encoded by these viruses. Our phylogenetic analyses clarified the taxonomic status of the obtained viral isolate. A rapid and cost-effective multiplex PCR method for simultaneous virus detection was developed. By testing the novel developed method using field samples, we found that CMV, PCV1 and BBWV2 were the three most common viruses. This research offers a comprehensive list of viruses affecting peppers in Guizhou and presents an efficient detection method, facilitating our understanding and practical management of pepper virus infections.

分类号：

  • 相关文献
作者其他论文 更多>>
  • MicrobiomeStatPlots: Microbiome statistics plotting gallery for meta-omics and bioinformatics

    作者：Bai, Defeng;Ma, Chuang;Xun, Jiani;Luo, Hao;Yang, Haifei;Lyu, Hujie;Gai, Anran;Yousuf, Salsabeel;Gao, Yunyun;Wang, Yao;Liu, Yong-Xin;Ma, Chuang;Yang, Haifei;Lyu, Hujie;Zhu, Zhihao;Gai, Anran;Peng, Kai;Xu, Shanshan;Gao, Yunyun

    关键词：bioinformatics; interpretation; microbiome; multi-omics; visualization

  • Identifications of Genes Involved in ABA and MAPK Signaling Pathways Positively Regulating Cold Tolerance in Rice

    作者：Ding, Guohua;Li, Zhugang;Cao, Liangzi;Zhou, Jinsong;Lei, Lei;Luo, Yu;Bai, Liangming;Yang, Guang;Sun, Shichen;Ding, Guohua;Li, Zhugang;Cao, Liangzi;Zhou, Jinsong;Lei, Lei;Luo, Yu;Bai, Liangming;Wang, Rongsheng;Li, Kun;Wang, Xueyang;Liu, Kai;Sun, Shichen;Iqbal, Zubair;Qu, Mingnan;Zhao, Minghui;Cui, Zhibo;Qu, Mingnan

    关键词：cold stress; ABA signaling; MAPK signaling; transcriptional regulation; integrative analysis

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词：metagenome; microbiome; microbiota; pipeline; visualization

  • The m6A Methylation Profile Identified That OsHMT9.1 Deregulates Chromium Toxicity in Rice (Oryza sativa L.) Through Negative Regulatory Functions

    作者：Hou, Yushan;Kong, Xuejiao;Li, Jingwen;Liu, Changsheng;Wang, Shuo;Wang, Jingguo;Liu, Hualong;Zheng, Hongliang;Xin, Wei;Zou, Detang;Yang, Luomiao;Xie, Shupeng;Wei, Zhonghua;Lei, Lei

    关键词：chromium stress; Oryza sativa L.; N6-methyladenosine (m6A) modification; RNA-Seq; OsHMT9.1

  • Analysis of metabolic differences in Tibetan medicinal plant Phlomoides rotata leaves in different habitats based on non-targeted metabolomics

    作者：Wang, Lele;Wang, Hongli;Chen, Junlin;Lamu, Yuzhen;Qi, Xiangyang;Lei, Lei;Mao, Kangshan;Tso, Sonam;Wang, Lele;Tso, Sonam;Wang, Hongli;Wang, Hongli;Mao, Kangshan

    关键词：Tibetan medicine; Phlomoides rotata; metabolomics; altitude gradient; slope direction; differential metabolites

  • Revealing the Effect of Non-rubber Components of Natural Rubber on Its Radiation Resistance Performance

    作者：Luo, En-Tao;Jing, Liang;Zhao, Li-Juan;Kong, Ling-Min;Xie, Zheng-Tian;Wu, Jin-Rong;Peng, Yu;Luo, Hao

    关键词：Natural rubber; Non-rubber components; Radiation resistance

  • Lignicolous freshwater fungi of the pan Qinghai-Xizang Plateau, China

    作者：Xu, Rong-Ju;Hyde, Kevin D.;Li, Jia-Ning;Li, Ying;Zhu, Xue-Tai;Li, Tai-Shun;Xu, Ke;Yu, Feng-Ming;Lu, Jin-Rong;Lei, Lei;Zhou, De-Qun;Yang, Zhu-Liang;Zhao, Qi;Xu, Rong-Ju;Hyde, Kevin D.;Boonmee, Saranyaphat;Liu, Ning-Guo;Li, Ying;Shen, Hong-Wei;Lei, Lei;Li, Jia-Ning;Zhu, Yin-An;Xu, Ke;Wu, Na;Liu, Ning-Guo;Lu, Yong-Zhong;Wu, Na;Liu, Jian-Kui;Shen, Hong-Wei;Luo, Zong-Long;Zhu, Xue-Tai;Wu, Dong-Mei;Gao, Neng;Hyde, Kevin D.;Al-Otibi, Fatimah;Bao, Dan-Feng;Jia, Pei-Song;He, Xiao-Lan;Yang, Jing

    关键词：49 new taxa; Asexual morph; Multi-gene; Phylogeny; Sexual morph; Taxonomy

微信小程序