Complete Genome Sequence of Macrobrachium rosenbergii Golda Virus (MrGV) from China

文献类型： 外文期刊

第一作者： Meng, Fanzeng

作者： Meng, Fanzeng;Wang, Guohao;Huang, Jie;Meng, Fanzeng;Wang, Yiting;Wang, Guohao;Tang, Kathy F. J.;Zhang, Fan;Dong, Xuan;Huang, Jie;Hu, Tao;Shi, Weifeng;Xu, La;Dong, Xuan;Zhang, Fan;Huang, Jie

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关键词： Macrobrachium rosenbergii; Macrobrachium rosenbergii golda virus; Goldavirus; Nidovirales; aquaculture

期刊名称：ANIMALS （ 影响因子：3.231； 五年影响因子：3.312 ）

ISSN： 2076-2615

年卷期： 2022 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Simple Summary Macrobrachium rosenbergii golda virus (MrGV) was first identified in Macrobrachium rosenbergii in Bangladesh with massive larva death. In this study, the variant of MrGV Mr-18 from China was accidentally found in a meta-transcriptome study of M. rosenbergii and compared with MrGV LH1-2018 reported in Bangladesh. Phylogenetic analysis has shown that these two variants belong to the same, yet unclassified, genus. At present, there has no evidence that MrGV Mr-18 causes disease in M. rosenbergii. However, we should be alert that MrGV may lead to the mass death of M. rosenbergii larvae; thus, surveillance of MrGV in Asia should be given priority. In a meta-transcriptome study of the giant freshwater prawn Macrobrachium rosenbergii sampled in 2018 from a hatchery, we identified a variant of Macrobrachium rosenbergii golda virus (MrGV) in postlarvae without clinical signs. The virus belongs to the family Roniviridae, and the genome of this MrGV variant, Mr-18, consisted of 28,957 nucleotides, including 4 open reading frames (ORFs): (1) ORF1a, encoding a 3C-like protein (3CLP) (4933 aa); (2) ORF1b, encoding a replicase polyprotein (2877 aa); (3) ORF2, encoding a hypothetical nucleocapsid protein (125 aa); and (4) ORF3, encoding a glycoprotein (1503 aa). ORF1a overlaps with ORF1b with 40 nucleotides, where a -1 ribosomal frameshift with slippage sequence 5 '-G(14925)GGUUUU(14931)-3 ' produces the pp1ab polyprotein. The genomic sequence of Mr-18 shared 97.80% identity with MrGV LH1-2018 discovered in Bangladesh. The amino acid sequence identities between them were 99.30% (ORF1a), 99.60% (ORF1b), 100.00% (ORF2), and 99.80% (ORF3), respectively. Phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) proteins revealed that they clustered together and formed a separate cluster from the genus Okavirus. The finding of MrGV in China warrants further studies to determine its pathogenicity and prevalence within the region.

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