A novel thermo-responsive phospholipase A 1 with high selectivity and efficiency in enzymatic oil degumming

文献类型： 外文期刊

第一作者： Li, Zhong

作者： Li, Zhong;Zhou, Huilin;Liu, Xuan;Lan, Dongming;Wang, Yonghua;Wang, Weifei;Wang, Yonghua

作者机构：

关键词： Phospholipase A 1; Enzymatic properties; Phospholipid substrate specificity; Enzymatic degumming; Phospholipid composition

期刊名称：FOOD CHEMISTRY （ 影响因子：8.5； 五年影响因子：8.2 ）

ISSN： 0308-8146

年卷期： 2024 年 456 卷

页码：

收录情况： SCI

摘要： The limited availability of phospholipase A1 (PLA1) has posed significant challenges in enzymatic degumming. In this study, a novel PLA1 (UM2) was introduced to address this limitation, which had a unique thermo-responsive ability to switch phospholipase and lipase activities in response to temperature variations. Remarkably, UM2 displayed an unprecedented selectivity under optimized conditions, preferentially hydrolyzing phospholipids over triacylglycerols-a specificity superior to that of commercial PLA1. Moreover, UM2 demonstrated high efficiency in hydrolyzing phospholipids with a predilection for phosphatidylcholine (PC) and phosphatidylethanolamine (PE). A practical application of UM2 on crude flaxseed oil led to a dramatic reduction in phosphorus content, plummeting from an initial 384.06 mg/kg to 4.38 mg/kg. Broadening its industrial applicability, UM2 effectively performed enzymatic degumming for other distinct crude vegetable oils with a unique phospholipid composition. Collectively, these results highlighted the promising application of UM2 in the field of oil degumming.

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