Orthologous genes Pm12 and Pm21 from two wild relatives of wheat show evolutionary conservation but divergent powdery mildew resistance

文献类型： 外文期刊

第一作者： Zhu, Shanying

作者： Zhu, Shanying;Zhu, Shanying;Chen, Zhaozhao;Liu, Tianlei;Liu, Renkang;Du, Haonan;Guo, Rui;He, Huagang;Liu, Cheng;Li, Genying;Gong, Shuangjun;Chen, Rong;Gao, Anli;Li, Miaomiao;Fan, Renchun;Liu, Zhiyong;Shen, Qian-Hua;Ma, Pengtao

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关键词： Pm12; Pm21; powdery mildew resistance; evolutionary conservation; race specificity; intramolecular interaction

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2023 年 4 卷 2 期

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收录情况： SCI

摘要： Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that threatens wheat production worldwide. Pm12, which originated from Aegilops speltoides, a wild relative of wheat, confers strong resistance to powdery mildew and therefore has potential use in wheat breeding. Using susceptible mutants induced by gamma irradiation, we physically mapped and isolated Pm12 and showed it to be orthologous to Pm21 from Dasypyrum villosum, also a wild relative of wheat. The resistance function of Pm12 was validated via ethyl methanesulfonate mutagenesis, virus-induced gene silencing, and stable genetic transformation. Evolutionary analysis indicates that the Pm12/Pm21 loci in wheat species are relatively conserved but dynamic. Here, we demonstrated that the two orthologous genes, Pm12 and Pm21, possess differential resistance against the same set of Bgt isolates. Overexpression of the coiled-coil domains of both PM12 and PM21 induces cell death in Nicotiana benthamiana leaves. However, their full-length forms display different cell death-inducing activities caused by their distinct intramolecular in-teractions. Cloning of Pm12 will facilitate its application in wheat breeding programs. This study also gives new insight into two orthologous resistance genes, Pm12 and Pm21, which show different race specificities and intramolecular interaction patterns.

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