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黄瓜SSR反应体系的建立

文献类型: 中文期刊

作者: 葛风伟 1 ; 张海英 2 ; 陈青君 3 ; 王永健 2 ; 许勇 2 ;

作者机构: 1.新疆农业大学园艺系

2.国家蔬菜工程技术研究中心

3.中国农业大学园艺系

关键词: 黄瓜;SSR;琼脂糖凝胶;聚丙烯酰胺凝胶

期刊名称: 华北农学报

ISSN: 1000-7091

年卷期: 2004 年 19 卷 02 期

页码: 9-13

收录情况: 北大核心 ; CSCD

摘要: 为摸索适宜黄瓜的SSR反应体系,分析了PCR反应体系中的Mg2+浓度、、dNTP浓度、DNA模板浓度、引物浓度、Taq聚合酶浓度对扩增结果的影响,并比较了琼脂糖凝胶和聚丙烯酰胺凝胶体系在检测扩增产物多态性方面的差异。结果表明:在25μL PCR反应体系中,Mg2+的最适浓度为0.2 mmol/L;dNTP最适浓度为0.2 mmol/L;反应体系中Taq聚合酶宜加入1U,引物应加入30 ng;DNA 最适浓度为 5 ng/μL。

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