文献类型： 外文期刊

作者： Li, Yu-Ze ¹ ; Zhu, Jia-Wei ¹ ; Lin, Wei ² ; Lan, Mo-Ying ¹ ; Luo, Cong ¹ ; Xia, Li-Ming ¹ ; Zhang, Yi-Li ¹ ; Liang, Rong-Zhen ¹ ; Hu, Wang-Li ¹ ; Huang, Gui-Xiang ¹ ; He, Xin-Hua ¹ ;

作者机构： 1.Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobi, 100 East Daxue Rd, Nanning 530004, Peoples R China

2.Fujian Acad Agr Sci, Inst Subtrop Agr, Zhangzhou 363005, Peoples R China

关键词： 'XiangShui' lemon; S-RNase; self-incompatibility; expression analysis; functional analysis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 18 期

页码：

收录情况： SCI

摘要： S-RNase plays vital roles in the process of self-incompatibility (SI) in Rutaceae plants. Data have shown that the rejection phenomenon during self-pollination is due to the degradation of pollen tube RNA by S-RNase. The cytoskeleton microfilaments of pollen tubes are destroyed, and other components cannot extend downwards from the stigma and, ultimately, cannot reach the ovary to complete fertilisation. In this study, four S-RNase gene sequences were identified from the 'XiangShui' lemon genome and ubiquitome. Sequence analysis revealed that the conserved RNase T2 domains within S-RNases in 'XiangShui' lemon are the same as those within other species. Expression pattern analysis revealed that S-3-RNase and S-4-RNase are specifically expressed in the pistils, and spatiotemporal expression analysis showed that the S-3-RNase expression levels in the stigmas, styles and ovaries were significantly higher after self-pollination than after cross-pollination. Subcellular localisation analysis showed that the S-1-RNase, S-2-RNase, S-3-RNase and S-4-RNase were found to be expressed in the nucleus according to laser confocal microscopy. In addition, yeast two-hybrid (Y2H) assays showed that S-3-RNase interacted with F-box, Bifunctional fucokinase/fucose pyrophosphorylase (FKGP), aspartic proteinase A1, RRP46, pectinesterase/pectinesterase inhibitor 51 (PME51), phospholipid:diacylglycerol acyltransferase 1 (PDAT1), gibberellin receptor GID1B, GDT1-like protein 4, putative invertase inhibitor, tRNA ligase, PAP15, PAE8, TIM14-2, PGIP1 and p24beta2. Moreover, S-3-RNase interacted with TOPP4. Therefore, S-3-RNase may play an important role in the SI of 'XiangShui' lemon.