Multifunctional nanoenzyme lateral flow immunoassay strip for rapid and ultrasensitive detection of carbofuran in vegetables
文献类型： 外文期刊
作者： Zhai, Shengxi 1 ; Dong, Haowei 1 ; Wang, Haifang 4 ; Huang, Jingcheng 1 ; Li, Donghan 1 ; Li, Zhengtao 1 ; Li, Zhaopeng 1 ; Li, Peisen 1 ; Zhang, Pengwei 1 ; Zhao, Mingxin 5 ; Sun, Xia 1 ; Guo, Yemin 1 ;
作者机构： 1.Shandong Univ Technol, Coll Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
2.Shandong Prov Engn Res Ctr Vegetable Safety & Qual, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
3.Zibo City Key Lab Agr Prod Safety Traceabil, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China
4.Beijing Univ Chinese Med, Dongzhimen Hosp, Beijing 100700, Peoples R China
5.Gansu Acad Agr Sci, Inst Fruit & Floriculture, Lanzhou 730070, Gansu, Peoples R China
关键词： Lateral flow immunoassay strip; Carbofuran; Nanoenzyme; Magnetic separation; Pesticide detection in vegetables
期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：12.2； 五年影响因子：11.9 ）
ISSN： 0304-3894
年卷期： 2024 年 477 卷
页码：
收录情况： SCI
摘要： A lateral flow immunoassay strip (LFIAS) is one of the most frequently rapid test technologies for carbofuran (CAR). Nevertheless, the LFIAS has a poor quantitative capability and low sensitivity. And, it also requires often complex sample handling steps, making testing time longer. In this study, Fe3O4 nanoparticles were successively modified with MIL-100(Fe)-based metal-organic framework (MOF) and chloroplatinic acid hexahydrate to obtain a core-shell complex of Fe3O4-MOF-Pt. The complex had a peroxidase-mimicking activity catalytic function that enabled signal amplification and sensitivity enhancement. Upon coupling with carbofuran monoclonal antibody (CAR-mAb), the magnetic separation properties of the probe enabled target-specific enrichment. The LFIAS based on Fe3O4-MOF-Pt nanocomposites could detect CAR in the range of 0.25-50 ng mL-1 with a limit of detection (LOD) of 0.15 ng mL-1, enabling colorimetric and catalytic analysis. In addition, the method showed high specificity and stability for detecting CAR in various vegetables, and recovery rates of the spiked samples were 91.40%-102.40%. In conclusion, this study provided one-stop detection of "target enrichment-visual inspection". While lowering the LOD, it reduced the detection time and improved the detection efficiency. The multifunctional Fe3O4-MOF-Pt nanocomposite provides an idea for the construction of novel multifunctional probes to improve the detection performance of conventional LFIAS.
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