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Characterization of the heat shock factor RcHsfA6 in Rosa chinensis and function in the thermotolerance of Arabidopsis

文献类型： 外文期刊

作者： Kang, Yanhui 1 ; Yang, Yuan 1 ; Sun, Pei 1 ; Li, Maofu 1 ; Wang, Hua 1 ; Sun, Xiangyi 1 ; Jin, Wanmei 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100093, Peoples R China

3.Engn Res Ctr Funct Floriculture, Beijing 100093, Peoples R China

4.Beijing Engn Res Ctr Deciduous Fruit Trees, Beijing 100093, Peoples R China

关键词： R. chinensis; Transcriptome; Heat shock transcription factor; RcHsfA6; ABA; Thermotolerance

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1471-2229

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundEnvironmental stresses, especially high temperatures, severely limit the growth and development of many horticultural plants. As a woody ornamental flower with rich flower colors and flower types, rose (R. chinensis) leaves wilt and shriveled petals at high temperatures, which severely affects its growth and ornamental value. The defense mechanism of rose plants against high-temperature stress has not been fully elucidated.ResultsIn the present study, the transcriptomes of rose petals at normal (25 degrees C) and high (35 degrees C) temperature were compared. A total of 2519 differentially expressed genes (DEGs) were identified, including 1491 upregulated DEGs and 1028 downregulated DEGs. The plant hormone signal transduction pathway, especially the abscisic acid (ABA) signaling pathway, was the most enriched signaling pathway for DEGs in rose at high temperature. Heat shock factors (Hsfs), especially class A Hsfs, have been confirmed to be involved in thermotolerance mechanisms. Among the DEGs, eight genes were annotated as Hsfs, including 5 upregulated Hsfs at high temperature. RcHsfA6 is rapidly induced by high temperatures and is a candidate regulatory factor in the plant ABA signaling pathway. Therefore, we focused on RcHsfA6. RcHsfA6 encodes a protein containing 308 amino acids and contains typical Hsf domains, such as the DNA-binding domain (DBD), the N-terminal oligomerization domain (OD), the nuclear localization signal (NLS) and AHA motifs at the C-terminal activator domain (CTAD). The heterologous overexpression of RcHsfA6 in Arabidopsis increased the thermotolerance of Arabidopsis seeds. In addition, RcHsfA6 overexpression increased the ABA content and the expression of ABA biosynthetic gene AtABI5 and signal transduction gene AtPYL12, thereby inhibiting the germination of Arabidopsis seeds under exogenous ABA conditions.ConclusionsTaken together, our results suggest that RcHsfA6 is involved in the high-temperature response of rose and its heterologous overexpression in Arabidopsis increased the thermotolerance of Arabidopsis at high temperatures via the ABA signaling pathway.

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