The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneum
文献类型： 外文期刊
作者： Shen, Qing-Qing 1 ; Wang, Tian-Ju 2 ; Wang, Jun-Gang 3 ; He, Li-Lian 1 ; Zhao, Ting-Ting 3 ; Zhao, Xue-Ting 1 ; Xie, Lin-Yan 1 ; Qian, Zhen-Feng 1 ; Wang, Xian-Hong 1 ; Liu, Lu-Feng 1 ; Chen, Shu-Ying 1 ; Zhang, Shu-Zhen 3 ; Li, Fu-Sheng 1 ;
作者机构： 1.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China
2.Chuxiong Normal Univ, Inst Bioresources Res & Dev Cent Yunnan Plateau, Chuxiong 675000, Yunnan, Peoples R China
3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
4.Yunnan Agr Univ, Key Lab Crop Prod & Smart Agr Yunnan Prov, Kunming 650201, Yunnan, Peoples R China
关键词： Sugarcane; Drought tolerance; WRKY transcription Factors; Protein interaction
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）
ISSN： 0981-9428
年卷期： 2023 年 199 卷
页码：
收录情况： SCI
摘要： In this study, we characterized a WRKY family member gene, SsWRKY1, which is located in the nucleus , contains multiple stress-related cis-acting elements. In addition, constructed SsWRKY1-overexpressing Arabi-dopsis thaliana had higher antioxidant enzyme activity and proline content under drought stress conditions, with lower malondialdehyde content and reactive oxygen species (ROS) accumulation , the expression levels of six stress-related genes were significantly upregulated. This indicates that the overexpression of SsWRKY1 in Ara-bidopsis thaliana improves resistance to drought stress. SsWRKY1 does not have transcriptional autoactivation activity in yeast cells. The yeast two-hybrid (Y2H) system and the S. spontaneum cDNA library were used to screen 21 potential proteins that interact with SsWRKY1, and the interaction between SsWRKY1 and ATAF2 was verified by GST pull-down assay. In summary, our results indicate that SsWRKY1 plays an important role in the response to drought stress and provide initial insights into the molecular mechanism of SsWRKY1 in response to drought stress.
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