文献类型： 外文期刊
作者： Deng, Fenglin 1 ; Zeng, Fanrong 1 ; Shen, Qiufang 2 ; Abbas, Asad 3 ; Cheng, Jianhui 1 ; Jiang, Wei 1 ; Chen, Guang 4 ; Shah, Adnan Noor 5 ; Holford, Paul 6 ; Tanveer, Mohsin 7 ; Zhang, Dabing 8 ; Chen, Zhong-Hua 6 ;
作者机构： 1.Yangtze Univ, Coll Agr, Collaborat Innovat Ctr Grain Ind, Jingzhou, Peoples R China
2.Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310058, Peoples R China
3.Anhui Agr Univ, Sch Hort, Hefei 230036, Peoples R China
4.Zhejiang Acad Agr Sci, Cent Lab, Hangzhou, Peoples R China
5.Khawaja Fareed Univ Engn & Informat Technol, Dept Agr Engn, Rahim Yar Khan 64200, Pakistan
6.Western Sydney Univ, Sch Sci, Penrith, NSW 2751, Australia
7.Univ Tasmania, Tasmanian Inst Agr, Hobart, Tas 7004, Australia
8.Shanghai Jiao Tong Univ, Joint Int Res Lab Metab & Dev Sci, Shanghai Jiao Tong Univ Univ Adelaide Joint Ctr A, Sch Life Sci & Biotechnol, Shanghai, Peoples R China
9.Univ Adelaide, Sch Agr Food & Wine, Glen Osmond, SA, Australia
10.Western Sydney Univ, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia
期刊名称：TRENDS IN PLANT SCIENCE （ 影响因子：22.012； 五年影响因子：23.116 ）
ISSN： 1360-1385
年卷期： 2022 年 27 卷 9 期
页码：
收录情况： SCI
摘要： Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic tools for medicine and life sciences. However, the application of this technology to miRNAs, with the function as negative gene regulators, has not been extensively reviewed in plants. Here, we summarize the evolution, biogenesis, and structure of miRNAs, as well as their interactions with mRNAs and computational models for predicting target genes. In addition, we review current advances in CRISPR/Cas for functional analysis and for modulating miRNA genes in plants. Extending our knowledge of miRNAs and their manipulation with CRISPR will provide fundamental understanding of the functions of plant miRNAs and facilitate more sustainable and publicly acceptable genetic engineering of crops.
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