Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes
文献类型： 外文期刊
作者： Yang, Lei 1 ; Xing, Binglin 1 ; Li, Fen 1 ; Wang, Li Kui 1 ; Yuan, Linlin 1 ; Mbuji, Amosi Leonard 1 ; Peng, Zhengqian 1 ;
作者机构： 1.Hainan Univ, Haikou, Hainan, Peoples R China
2.China Agr Univ, Coll Resources & Environm Sci, Dept Resources Utilizat & Plant Protect, Beijing, Peoples R China
3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Trop Crops, Haikou, Hainan, Peoples R China
4.Agr Res Ctr, Pesticide Residues & Environm Pollut Dept, Giza, Egypt
关键词： Spodoptera frugiperda; Resistance; Full-length transcriptome; Detoxification gene
期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）
ISSN： 2167-8359
年卷期： 2021 年 9 卷
页码：
收录情况： SCI
摘要： Background: Spodoptera frugiperda (J. E. Smith), commonly known as fall armyworm (FAW), is one of the most destructive agricultural pests in the world and has posed a great threat to crops. The improper use of insecticides has led to rapid development of resistance. However, the genetic data available for uncovering the insecticide resistance mechanisms are scarce. Methods: In this study, we used PacBio single-molecule real-time (SMRT) sequencing aimed at revealing the full-length transcriptome profiling of the FAW larval brain to obtain detoxification genes. Results: A total of 18,642 high-quality transcripts were obtained with an average length of 2,371 bp, and 11,230 of which were successfully annotated in six public databases. Among these, 5,692 alternative splicing events were identified.
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