Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize
文献类型： 外文期刊
作者： Wang FengGe 1 ; Zhao JiuRan 2 ; Dai JingRui 2 ; Yi HongMei 2 ; Kuang Meng 2 ; Sun YanMei 2 ; Yu XinYan 3 ; Guo JingLun; W 1 ;
作者机构： 1.China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100094, Peoples R China
2.China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100094, Peoples R China; Beijing Acad Agr & Forestry Sci, Maize Res Ctr, Beijing 100097, Peoples R China
3.China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100094, Peoples R China; Beijing Acad Agr & Forestry Sci, Maize
关键词： maize;core primer;SSR;multiplex PCR;capillary electrophoresis;fluorescent-labelled
期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）
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收录情况： SCI
摘要： In the current study, 1900 maize simple sequence repeat (SSR) primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer per chromosome.
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