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Characterization of DnaJ gene family in Castanea mollissima and functional analysis of CmDnaJ27 under cold and heat stresses

文献类型： 外文期刊

作者： Yu, Liyang 1 ; Tian, Yujuan 1 ; Wang, Jinxin 2 ; Wang, Dongsheng 1 ; Wang, Xuan 1 ; Zhang, Haie 1 ; Zhang, Jingzheng 1 ; Wang, Xiangyu 3 ;

作者机构： 1.Hebei Normal Univ Sci & Technol, Engn Res Ctr Chestnut Ind Technol, Minist Educ, Qinhuangdao 066004, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Shijiazhuang Inst Pomol, Shijiazhuang 050061, Hebei, Peoples R China

3.Hebei Normal Univ Sci & Technol, Off Sci Res, Qinhuangdao 066004, Peoples R China

关键词： DnaJ gene family; Castanea mollissima; Expression analysis; RT-qPCR; Subcellular location; Functional validation

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1471-2229

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundThe DnaJ gene family plays crucial roles in the plant abiotic stress response. Although the DnaJ gene family has been extensively characterized in various plants, its distribution in the Castanea mollissima genome is still unclear.ResultsIn this study, 78 CmDnaJ genes were identified and characterized in the C. mollissima genome, divided into three subfamilies based on the phylogeny analysis. The abundance of cis-acting elements related to temperature stress in the promoter regions of the CmDnaJ genes suggested a close relationship between this family and temperature stress response. Dispersed duplication was identified as the main driving force behind the expansion of the CmDnaJ family. RNA-seq data from 192 runs across nine projects were analyzed, revealing the potential roles of CmDnaJ genes in the growth, development, and environmental stress response of C. mollissima. Weighted gene co-expression network analysis showed that CmDnaJ27 and CmDnaJ34 were located in the red module and significantly correlated with temperature stimulus-response. RT-qPCR experiments and subcellular localization validated the expression levels and specific locations of CmDnaJ genes under cold and heat stresses. Overexpression of CmDnaJ27 in Nicotiana tabacum significantly reduces its tolerance to cold and heat stresses.ConclusionsThis study provides insights into the important roles of the DnaJ gene family in C. mollissima growth, development, and response to environmental stress, and provides reference to the research of CmDnaJs in cold and heat stresses response.

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