Development of a multienzyme isothermal and lateral flow dipstick combination assay for the rapid detection of goose astrovirus II
文献类型： 外文期刊
作者： Zhu, Yinchu 1 ; Chen, Liu 1 ; Xu, Xin 1 ; Ye, Weicheng 1 ; Ni, Zheng 1 ; Huo, Suxin 1 ; Hua, Jionggang 1 ; Yun, Tao 1 ; Yao, Huochun 2 ; Wang, Hongyu 1 ; Zhang, Cun 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China
2.Nanjing Agr Univ, Coll Vet Med, Nanjing, Peoples R China
关键词： goose astrovirus; multienzyme isothermal rapid amplification; lateral flow strip; rapid detection; clinic diagnostic
期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：4.6； 五年影响因子：5.1 ）
ISSN： 2235-2988
年卷期： 2024 年 14 卷
页码：
收录情况： SCI
摘要： Introduction Goose astrovirus (GAstV) is a newly emerging pathogen that is currently widespread among geese, causing visceral gout and leading to substantial gosling mortalities, posing a severe threat to the waterfowl industry. GAstV II is the predominant epidemic strain, characterized by its high morbidity and mortality rate. Consequently, there is an urgent necessity to develop an effective diagnostic approach to control the dissemination of GAstV II, particularly in clinical farms with limited laboratory resources.Methods In this study, a novel multi-enzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD) combined assay was developed. Different primers designed specific targeting a highly conserved region within the viral RdRp gene for the detection of GAstV II. Primers optimized and MIRA-LFD assay analyzed its performance regarding limits of detection, specificity, and efficiency of detection.Results The developed MIRA amplification is conducted at a constant temperature and accomplished within 10 minutes. Subsequent naked-eye observation of the LFD strips merely takes 5 minutes. The established MIRA-LFD method exhibits high specificity, with no cross-reaction with other pathogens and attains a detection sensitivity of 1 copy/mu l, which is consistent with the reverse transcription quantitative PCR (RT-qPCR) assay. Further evaluation with clinical samples indicates that the accuracy of this MIRA-LFD method correlates well with RT-qPCR for the detection of GAstV II.Conclusion In summary, the convenience, sensitivity, and rapidity of this newly developed detection method offer a significant advantage for on-site diagnosis of GAstV II.
- 相关文献
作者其他论文 更多>>
-
Complete Genome Sequence and Construction of an Infectious Bacterial Artificial Chromosome Clone of a Virulent Duck Enteritis Virus Strain XJ
作者：Huo, Su-xin;Bao, En-dong;Huo, Su-xin;Zhu, Yin-chu;Chen, Liu;Yun, Tao;Ye, Wei-cheng;Hua, Jiong-gang;Ni, Zheng;Xiang, Sheng-rui;Ding, Fang-zhou;Gao, Xu;Zhang, Cun;Xiang, Sheng-rui;Ding, Fang-zhou;Gao, Xu;Liu, Han-bin
关键词：
-
High expression of the classical swine fever virus (CSFV) envelope protein E2 by a single amino acid mutation and its embedded in the pseudorabies virus (PRV) vector for immunization
作者：Sun, Yang-yang;Liu, Ke-shu;Yun, Tao;Ni, Zheng;Zhu, Yin-chu;Chen, Liu;Ye, Wei-cheng;Hua, Jiong-gang;Huo, Su-xin;Wang, Hong-yu;Zhang, Cun;Sun, Yang-yang;Bao, En-dong;Bao, Hai-li
关键词：Pseudorabies virus; Classical swine fever; Bivalent vaccine; A transmembrane region; E2 protein
-
Inactivation Performance of Pseudorabies Virus as African Swine Fever Virus Surrogate by Four Commercialized Disinfectants
作者：Ni, Zheng;Chen, Liu;Yun, Tao;Ye, Weicheng;Hua, Jionggang;Zhu, Yinchu;Zhang, Cun;Xie, Ronghui
关键词：evaluation model for disinfectant; inactivation performance; infection control; African swine fever virus (ASFV); pseudorabies virus (PRV)
-
Study on the Protective Immunity Induced by Pseudotyped Baculovirus Expressing the E Protein of Tembusu Virus in Ducklings
作者：Ni, Zheng;Yun, Tao;Chen, Liu;Ye, Weicheng;Hua, Jionggang;Zhu, Yinchu;Zhang, Cun;Liu, Guangqing
关键词：DTMUV; E protein; protection; recombinant baculovirus; subunit vaccine
-
Recombinant pseudorabies virus (PRV) expressing stabilized E2 of classical swine fever virus (CSFV) protects against both PRV and CSFV
作者：Sun, Yang-yang;Bao, En-dong;Sun, Yang-yang;Liu, Ke-shu;Zhang, Cun;Ni, Zheng;Zhu, Yin-chu;Chen, Liu;Ye, Wei-cheng;Hua, Jiong-gang;Huo, Su-xin;Wang, Hong-yu;Yun, Tao;Yun, Tao;Bao, Hai-li
关键词：Pseudorabies virus; Classical swine fever; Bivalent vaccine; Energy; Stabilized E2 protein
-
Porcine extraintestinal pathogenic Escherichia coli delivers two serine protease autotransporters coordinately optimizing the bloodstream infection
作者：Pan, Xinming;Chen, Rong;Zhang, Yating;Yao, Huochun;Ma, Jiale;Pan, Xinming;Chen, Rong;Zhang, Yating;Yao, Huochun;Ma, Jiale;Pan, Xinming;Chen, Rong;Zhang, Yating;Yao, Huochun;Ma, Jiale;Zhu, Yinchu;Zhao, Jin
关键词：extraintestinal pathogenic E; coli; bloodstream; serine protease autotransporter; immunomodulation; mucin-like glycoprotein
-
Distinct Whole Transcriptomic Profiles of the Bursa of Fabricius in Muscovy Ducklings Infected by Novel Duck Reovirus with Different Virulence
作者：Yun, Tao;Hua, Jionggang;Ni, Zheng;Ye, Weicheng;Chen, Liu;Zhu, Yinchu;Zhang, Cun
关键词：novel duck reovirus; bursa of Fabricius; whole transcriptomic analysis; miRNA
