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Aspartyl proteases identified as candidate genes of a fiber length QTL, qFLD05, that regulates fiber length in cotton (Gossypium hirsutum L.)

文献类型： 外文期刊

作者： Zhang, Sujun 1 ; Wang, Haitao 1 ; Li, Xinghe 1 ; Tang, Liyuan 1 ; Cai, Xiao 1 ; Liu, Cunjing 1 ; Zhang, Xiangyun 1 ; Zhang, Jianhong 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Cotton, Key Lab Biol & Genet Improvement Cotton Huanghuai, Minist Agr & Rural Affairs, Shijiazhuang 050051, Hebei, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2024 年 137 卷 3 期

页码：

收录情况： SCI

摘要： Key message GhAP genes were identified as the candidates involved in cotton fiber length under the scope of fine mapping a stable fiber length QTL, qFL(D05). Moreover, the transcription factor GhWRKY40 positively regulated GhAP3 to decrease fiber length. Fiber length (FL) is an economically important fiber quality trait. Although several genes controlling cotton fiber development have been identified, our understanding of this process remains limited. In this study, an FL QTL (qFL(D05)) was fine-mapped to a 216.9-kb interval using a secondary F-2:3 population derived from the upland hybrid cultivar Ji1518. This mapped genomic segment included 15 coding genes, four of which were annotated as aspartyl proteases (GhAP1-GhAP4). GhAPs were identified as candidates for qFL(D05) as the sequence variations in GhAPs were associated with FL deviations in the mapping population, and functional validation of GhAP3 and GhAP4 indicated a longer FL following decreases in their expression levels through virus-induced gene silencing (VIGS). Subsequently, the potential involvement of GhWRKY40 in the regulatory network was revealed: GhWRKY40 positively regulated GhAP3's expression according to transcriptional profiling, VIGS, yeast one-hybrid assays and dual-luciferase experiments. Furthermore, alterations in the expression of the eight previously reported cotton FL-responsive genes from the above three VIGS lines (GhAP3, GhAP4 and GhWRKY40) implied that MYB5_A12 was involved in the GhWRKY40-GhAP network. In short, we unveiled the unprecedented FL regulation roles of GhAPs in cotton, which was possibly further regulated by GhWRKY40. These findings will reveal the genetic basis of FL development associated with qFL(D05) and be beneficial for the marker-assisted selection of long-staple cotton.

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