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Comparative Analysis of miRNA Expression Profiles under Salt Stress in Wheat

文献类型： 外文期刊

作者： Qiao, Hualiang 1 ; Jiao, Bo 1 ; Wang, Jiao 1 ; Yang, Yang 1 ; Yang, Fan 1 ; Geng, Zhao 2 ; Zhao, Guiyuan 2 ; Liu, Yongwei 1 ; Dong, Fushuang 1 ; Wang, Yongqiang 2 ; Zhou, Shuo 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Plant Genet Engn Ctr Hebei Prov, Inst Biotechnol & Food Sci, Shijiazhuang 050051, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Cotton, Shijiazhuang 050051, Peoples R China

关键词： wheat; salt stress; miRNA; high-throughput sequencing

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 8 期

页码：

收录情况： SCI

摘要： Salt stress is one of the important environmental factors that inhibit the normal growth and development of plants. Plants have evolved various mechanisms, including signal transduction regulation, physiological regulation, and gene transcription regulation, to adapt to environmental stress. MicroRNAs (miRNAs) play a role in regulating mRNA expression. Nevertheless, miRNAs related to salt stress are rarely reported in bread wheat (Triticum aestivum L.). In this study, using high-throughput sequencing, we analyzed the miRNA expression profile of wheat under salt stress. We identified 360 conserved and 859 novel miRNAs, of which 49 showed considerable changes in transcription levels after salt treatment. Among them, 25 were dramatically upregulated and 24 were downregulated. Using real-time quantitative PCR, we detected significant changes in the relative expression of miRNAs, and the results showed the same trend as the sequencing data. In the salt-treated group, miR109 had a higher expression level, while miR60 and miR202 had lower expression levels. Furthermore, 21 miRNAs with significant changes were selected from the differentially expressed miRNAs, and 1023 candidate target genes were obtained through the prediction of the website psRNATarget. Gene ontology (GO) analysis of the candidate target genes showed that the expressed miRNA may be involved in the response to biological processes, molecular functions, and cellular components. In addition, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis confirmed their important functions in RNA degradation, metabolic pathways, synthesis pathways, peroxisome, environmental adaptation, global and overview maps, and stress adaptation and the MAPK signal pathway. These findings provide a basis for further exploring the function of miRNA in wheat salt tolerance.

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